1. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pX330_EZH2gRNA14

   Plasmid

   #199586

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330_EZH2gRNA15

   Plasmid

   #199587

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330_EZH2gRNA16

   Plasmid

   #199588

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330_EZH2gRNA17

   Plasmid

   #199589

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330-sgRNA_Dicer_2

   Plasmid

   #68808

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330-sgRNA_Dicer_1

   Plasmid

   #68807

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330-sgRNA_Dicer_3

   Plasmid

   #68809

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTP63.1.0-gDNA

    Plasmid

    #132432

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TP63
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTSHZ1.1.0-gDNA

    Plasmid

    #132434

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TSHZ1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDLX4.1.0-gDNA

    Plasmid

    #132437

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    DLX4
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZNF319.1.0-gDNA

    Plasmid

    #132438

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF319
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTHAP7.1.0-gDNA

    Plasmid

    #132439

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    THAP7
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZNF3.1.0-gDNA

    Plasmid

    #132440

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZNF551.1.0-gDNA

    Plasmid

    #132441

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF551
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBD1.1.0-gDNA

    Plasmid

    #132444

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    MBD1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge