Addgene

We narrowed to 2,825 results for: KIS

Showing: 91 - 100 of 2825 results

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pOC3 smFP-HA-Cacna1e KI

Plasmid

#183436

Purpose

CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.5’VIM-eGFP-KI

Plasmid

#170547

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for VIM knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pOC2 CRISPIE-Halo-Homer1 KI

Plasmid

#183446

Purpose

CreON knock-in for Halo-Homer1 (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and CRISPIE Halo donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Degron-KI ezh2 donor vector

Plasmid

#65620

Purpose

Donor vector to generate Degron-KI DD-EZH2

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

EZH2 left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pORANGE CACNG8-GFP KI #1

Plasmid

#131473

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pORANGE_NFL linker(A6TAG)-3xFLAG KI

Plasmid

#182680

Purpose

Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and chicken beta-actin promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Kcnn2-smFP-myc KI

Plasmid

#183438

Purpose

FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP myc donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 smFP-HA-Cacna1e KI

Plasmid

#183441

Purpose

FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Grin1-smFP-V5 KI

Plasmid

#183439

Purpose

FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP V5 donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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