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Showing: 271 - 300 of 7057 results

  1. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid
    #186305
    Purpose
    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    P3-PDGFR
    iRFP670
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186305-plasmid-map-sequence-id-366925

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  4. EF1a_MYC deltaLZ_P2A_Hygro_Barcode

    Plasmid
    #120509
    Purpose
    Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaLZ (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of leucine zipper motif
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120509-plasmid-map-sequence-id-234932

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  5. EF1a_MYC deltaHLH_P2A_Hygro_Barcode

    Plasmid
    #120508
    Purpose
    Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaHLH (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of helix-loop-helix motif
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120508-plasmid-map-sequence-id-234930

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  9. pEF1a-Foxd3-ER

    Plasmid
    #37270
    Depositor
    Patricia Labosky
    Insert
    Foxd3 (Foxd3 Mouse)
    Use
    Tags
    ER
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Aug. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  11. psLIB EF1alpha

    Plasmid
    #229954
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229954-plasmid-map-sequence-id-461280

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  14. pPB_EF1a_Gag-EIF4E-TM

    Plasmid
    #212658
    Purpose
    piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Piggybac
    Tags
    EIF4E (W56L, W102L, W166L)
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212658-plasmid-map-sequence-id-420239

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  28. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

    Plasmid
    #194462
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ΞΆ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194462-plasmid-map-sequence-id-384265

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  29. pKDR-EF1a.13

    Plasmid
    #183576
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 13
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183576-plasmid-map-sequence-id-370309

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  30. pKDR-EF1a.10

    Plasmid
    #183573
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 10
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183573-plasmid-map-sequence-id-365381

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Showing: 271 - 300 of 7057 results