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pKDR-EF1a.2

Plasmid

#183569

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 2

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

Plasmid

#194463

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.gp91.EF1aIntron.eGFP

Plasmid

#30466

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/EF1aIntron/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2Y641,645,646F

Plasmid

#22638

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 Y641,645,646F (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 641, 645 and 646 to Phenylalanine

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
EF1a_DIO_R133C-HaloTag

Plasmid

#164064

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 R133C (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-1-OsEf1a-6

Plasmid

#197703

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsEf1a promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmEf1a-6

Plasmid

#197712

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmEf1a promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-OsEf1a-11

Plasmid

#197766

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsEf1a terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-ZmEf1a-11

Plasmid

#197775

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmEf1a terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1aMS2p65HSF1hyg-W

Plasmid

#112920

Purpose

Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

MS2p65HSF1-T2Ahyg

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A1_GTP-EFTU-D2

Plasmid

#109811

Purpose

Protein expression and purification of EEF1A1_GTP-EFTU-D2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A1_GTP-EFTU-D2 (EEF1A1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-NLuc

Plasmid

#135938

Purpose

Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaST1

Plasmid

#22632

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST1 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST1 domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pRRL.CMVenh.gp91.EF1aIntron.eGFP

Plasmid

#30469

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91.EF1alntron.eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaST2

Plasmid

#22633

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST2 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST2 domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pEF1a-Foxd3-ER

Plasmid

#37270

Depositor

Patricia Labosky

Insert

Foxd3 (Foxd3 Mouse)

Tags

ER

Expression

Mammalian

Promoter

EF1

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
psLIB EF1alpha

Plasmid

#229954

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-P-hEF1a

Plasmid

#229163

Purpose

lvl 0 promoter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

hEf1a

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-VSVG

Plasmid

#212646

Purpose

piggyBac vector to express FLAG-VSVG

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

VSVG

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_HA-tNGFR_ZeoR

Plasmid

#212650

Purpose

piggyBac vector to express HA-tNGFR with zeocin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-tNGFR_PuroR

Plasmid

#212651

Purpose

piggyBac vector to express FLAG-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-4

Plasmid

#221490

Purpose

NKX2-4 lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-4 (NKX2-4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-5

Plasmid

#221491

Purpose

NKX2-5 lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS

Plasmid

#213393

Purpose

uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-SlEf1a-6

Plasmid

#197707

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

SlEf1a promoter

Expression

Plant

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-OsEf1a-4

Plasmid

#197696

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsEf1a promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-NtEf1a-6

Plasmid

#197702

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

NtEf1a promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-NtEf1a-11

Plasmid

#197764

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

NtEf1a terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-SlEf1a-11

Plasmid

#197771

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

SlEf1a terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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