A Single Transcript Knockdown-Replacement Strategy Employing 5' UTR Secondary Structures to Precisely Titrate Rescue Protein Translation.
Millette MM, Holland ED, Tenpas TJ, Dent EW
Front Genome Ed. 2022 Mar 28;4:803375. doi: 10.3389/fgeed.2022.803375. eCollection 2022. PubMed Article
Millette MM, Holland ED, Tenpas TJ, Dent EW
Front Genome Ed. 2022 Mar 28;4:803375. doi: 10.3389/fgeed.2022.803375. eCollection 2022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183553 | shRNA-Ratio-PosCon | Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2) |
| 183554 | shRNA-Ratio-NegCon | Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2) |
| 183555 | pKDR-Lenti-TetOn | pKDR compatible TetOn lentiviral transfer plasmid |
| 183557 | pKDR-CBh | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter) |
| 183558 | pKDR-CBh.4 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183559 | pKDR-CBh.6 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183560 | pKDR-CBh.8 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183561 | pKDR-CBh.10 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183562 | pKDR-CBh.13 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183563 | pKDR-CBh.14 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183564 | pKDR-CBh.18 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183565 | pKDR-CBh.21 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183566 | pKDR-CBh.22 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3) |
| 183567 | pKDR-EF1a | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter) |
| 183568 | pKDR-EF1a.1 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183569 | pKDR-EF1a.2 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183570 | pKDR-EF1a.3 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183571 | pKDR-EF1a.4 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183572 | pKDR-EF1a.5 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183573 | pKDR-EF1a.10 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183574 | pKDR-EF1a.11 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183575 | pKDR-EF1a.12 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183576 | pKDR-EF1a.13 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3) |
| 183577 | pKDR-TetOn | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter) |
| 183578 | pKDR-TetOn.1 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3) |
| 183579 | pKDR-TetOn.4 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3) |
| 183580 | pKDR-TetOn.5 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3) |
| 183581 | pKDR-TetOn.7 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3) |
| 183582 | pKDR-TetOn.9 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3) |
| 183583 | pKDR-TetOn.11 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3) |
| 183584 | pKDR-TetOn.13 | One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3) |
| 188460 | pKDR-Lenti-CBh.6-SOD1 | For knockdown-replacement of SOD1 gene (SOD1 KDR lentiviral transfer plasmid, Fig 4) |