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Plasmid#201644PurposeOver-expression of 3xFLAG-tagged human UPP1DepositorInsertUPP1 (UPP1 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI-UPP2-3xFLAG
Plasmid#201645PurposeOver-expression of 3xFLAG-tagged human UPP2DepositorInsertUPP2 (UPP2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-SHP1mut-cFlag
Plasmid#220208PurposeMammalian expression of TEX264 with SHP domain mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.280GEGPL>RERPA; c.838G>C,844G>A,850T&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-EGFP-Actin5c
Plasmid#15312DepositorInsertActin5c (Act5C Fly)
UseTagsEGFP and HisExpressionInsectMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TUBG1
Plasmid#171967PurposepGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion proteinDepositorInsertTUBG1 (TUBG1 Human)
UseTagsGSTExpressionBacterialMutationR275H (Please see depositor comments)PromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF1122
Plasmid#143467PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNRNPAB (HNRNPAB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1123
Plasmid#143764PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNRNPAB (HNRNPAB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCFD3-Act5c
Plasmid#130278PurposeDrosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.DepositorInsertpCFD3-Act5c (Act5C Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SERPINB5_WT
Plasmid#82898PurposeGateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.DepositorInsertSERPINB5 (SERPINB5 Human)
UseGateway entry vectorTagsExpressionMutationS176P; AA190-192delTDTins25;AA194-196delPVQinsSIP…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TH1202-pOCC175_pOEM1-N-HIS-MBP-PS-mGFP-TEV-HNRNPA3_opt
Plasmid#221890PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertHNRNPA3 (HNRNPA3 Human)
UseTags6xHis-MBP-PreScission-mGFP-TEVExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SERPINB5_p.I159S
Plasmid#82734PurposeGateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.DepositorInsertSERPINB5 (SERPINB5 Human)
UseGateway entry vectorTagsExpressionMutationI159S; S176P; p.190_192delTDTins25amino acids; p.…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SERPINB5_p.D141A
Plasmid#82735PurposeGateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.DepositorInsertSERPINB5 (SERPINB5 Human)
UseGateway entry vectorTagsExpressionMutationp. D141A; p. 176S>P; p.190_192delTDTins25amino…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SERPINB5_p.A7T
Plasmid#82736PurposeGateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.DepositorInsertSERPINB5 (SERPINB5 Human)
UseGateway entry vectorTagsExpressionMutationp. A7T; p.176S>P; p. 190_192delTDTins25; p.194…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SERPINB5_p.A42F
Plasmid#82738PurposeGateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.DepositorInsertSERPINB5 (SERPINB5 Human)
UseGateway entry vectorTagsExpressionMutationp.A42F; p.176S>P; p.190_192delTDTins25; p.194_…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only