We narrowed to 5,415 results for: 623

1. pGS1623

   Plasmid

   #63795

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   tDimer2-Twin-Strep tag followed by HIS5
   

   Use

   E. coli cloning vector

   Tags

   tDimer2-Twin-Strep

   Expression

   Mutation

   Promoter

   none

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. KHBD00623

   Plasmid

   #39695

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG17594 (scro Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pRL623

   Plasmid

   #58494

   Purpose

   helper plasmid for bacterial conjugal DNA transfer

   Depositor

   Peter Wolk

   Article

   Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

   Insert

   encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH623

   Plasmid

   #171633

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-non-targeting
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0623

   Plasmid

   #142666

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   DDIT3 (DDIT3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2623

   Plasmid

   #144053

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CRTC1 (CRTC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMM0623

   Plasmid

   #128998

   Purpose

   pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1623

   Plasmid

   #143142

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF510 (ZNF510 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDN623

   Plasmid

   #175377

   Purpose

   Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.

   Depositor

   Daniel Nickerson

   Article

   Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK623

    Plasmid

    #72540

    Purpose

    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGS1623

    Plasmid

    #63795

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    tDimer2-Twin-Strep tag followed by HIS5
    

    Use

    E. coli cloning vector

    Tags

    tDimer2-Twin-Strep

    Expression

    Mutation

    Promoter

    none

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KHBD00623

    Plasmid

    #39695

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17594 (scro Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pSL2623 (pCas6_Vch)

    Plasmid

    #200895

    Purpose

    Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046233

    Plasmid

    #149032

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28b-AF0623

    Plasmid

    #30164

    Depositor

    SeWon Suh

    Article

    Kim do et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    AF0623 (lig Archaeoglobus fulgidus)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pGBW-m4046230

    Plasmid

    #145454

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGEM-3z/623

    Plasmid

    #26664

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pGBW-m4046231

    Plasmid

    #145451

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046238

    Plasmid

    #145483

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGBW-m4133623

    Plasmid

    #152320

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;TRX

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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