Addgene

We narrowed to 4,849 results for: FU

Showing: 181 - 200 of 4849 results

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FUS_Halo_N_allele_400

Plasmid

#161767

Purpose

Donor vector for endogenous tagging of human FUS at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2olig-BFP

Plasmid

#155355

Purpose

Lentivector to express CRY2olig-BFP control

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

cry2olig-BFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_C_allele_400

Plasmid

#161768

Purpose

Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSupAR-MbPylRS(DiZPK)

Plasmid

#91705

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

Depositor

P. Chen

Article

Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

Inserts

pylS
pylT

Use

Tags

Expression

Bacterial

Mutation

T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

Promoter

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBS U3-hTR-500

Plasmid

#28170

Depositor

Kathleen Collins

Article

Fu et al Mol Cell. 2003 May . 11(5):1361-72.

Insert

U3-hTR-500 (TERC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-GFP

Plasmid

#86441

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

mCherry-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

mCherry-sfGFP

Expression

Mutation

Promoter

Plac

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLacZa-GFP

Plasmid

#86440

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

LacZalpha-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

LacZa-sfGFP

Expression

Mutation

Promoter

Plac

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF3-N

Plasmid

#155353

Purpose

Express human YTHDF3(1-385) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF3(1-385)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-C2

Plasmid

#155350

Purpose

Express human YTHDF1(360-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (360-559)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-C1

Plasmid

#155349

Purpose

Express human YTHDF1(264-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (262-559)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF2-N

Plasmid

#155352

Purpose

Express human YTHDF2(1-381) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF2(1-381)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-N

Plasmid

#155351

Purpose

Express human YTHDF1(1-359) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (1-359)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge_RtoK

Plasmid

#139127

Purpose

express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add K and D to FUS LC so it has the same charge p…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211462

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-GFP1-10-IRES-Puro

Plasmid

#211467

Purpose

Lentivirus backbone expressing GFP1-10

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

GFP1-10

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLID211A-HA

Plasmid

#211459

Purpose

Express mitochondrial localized ApaLI with D211A mutation

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-D211A

Use

Tags

HA

Expression

Mammalian

Mutation

D211A

Promoter

CMV

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLI*-HA

Plasmid

#211461

Purpose

Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI mutant

Use

Tags

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

CMV

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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