... them to a tumor - they used focused ultrasound (FUS) to heat the tumor area, as the image below shows...stable levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made...

...events that occur in less that 5% of the population (Fu et al. 2013, Vouillot et al. 2015). Meanwhile, Sanger...sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al. 2013). This approach...magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target mutagenesis...

...guides (19-20bp)?  Small-scale studies (Tsai et al., Fu et al.) suggest 17-19 bp long guides have reduced...PMID: 25513782. Pubmed Central PMCID: PMC4320685. 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease specificity...

...However, Cas9 can be toxic if continuously expressed (Fu et al., 2013). Using IDLVs ensures transient expression...94-007-4899-6_7. PubMed Central PMCID: PMC7120651 Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon...

...Yardena Samuels 29591 426Gal-FUS FUS GAL1 ALS Aaron Gitler 29592 426Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler...Gitler 29593 416Gal-FUS-YFP FUS YFP GAL1 ALS Aaron Gitler 29594 416GPD-FUS-YFP FUS YFP GPD ALS Aaron Gitler...416Gal-SV40NLS-FUS-YFP FUS YFP, SV40 NLS GAL1 ALS Aaron Gitler 29596 426Gal-FUS-1-170aa-YFP FUS YFP GAL1 ALS...29597 426Gal-FUS-1-270aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29598 426Gal-FUS-1-373aa-YFP FUS YFP GAL1 ALS...29599 426Gal-FUS-1-413aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29600 426Gal-FUS-1-453aa-YFP FUS YFP GAL1 ALS...29601 426Gal-FUS-1-501aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29603 426Gal-FUS-101-526aa-YFP FUS YFP GAL1 ALS...29605 426Gal-FUS-267-526aa-YFP FUS YFP GAL1 ALS Aaron Gitler 29606 426Gal-FUS-285-526aa-YFP FUS YFP GAL1 ...

...PMID: 26829887. PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al. High-frequency off-target mutagenesis ...

...10.1016/j.neuron.2010.11.021  Gambarotto D, Zwettler FU, Guennec ML, et al (2019) Imaging cellular ultrastructures...

...Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules...

...Structural Genomics Consortium (SGC) Plasmids Haian Fu Lab plasmids Opher Gileadi Lab plasmids (Link opens...

...modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has ...

...Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell...

...him on Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman PS, Fire AZ (2014) Efficient...

...://doi.org/10.1016/j.tibs.2023.10.006 Tang, Y., & Fu, Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology...

...one of the following genes: SOD1, C9ORF72, TARDBP, FUS and OPTN. Huntington's Disease Huntington’s disease...

...Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. 2014....

...Viral delivery methods include focused ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV...

...Molecular Cell , 73 (4), 714-726.e4. PMID: 30581144 Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon...Nature Biotechnology , 31 (9), 822–826. PMID: 23792628 Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung...

...Molecular Cell , 73 (4), 714-726.e4. PMID: 30581144 Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon...Nature Biotechnology , 31 (9), 822–826. PMID: 23792628 Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung...