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Plasmid#213333PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertLTB4R (LTB4R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCA2-DuET
Plasmid#213308PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHCA2 (HCAR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPBA-DuET
Plasmid#213255PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPBA (GPBAR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC52A1
Plasmid#132169PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC52A1 (SLC52A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFB-CG-LYCHOS WT-HA-GFP
Plasmid#199687PurposeExpression of LYCHOS WT in insect cellsDepositorInsertLYCHOS (GPR155 Human)
UseTags10xHis, EGFP, HA, HRV 3C, and HisExpressionInsectMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED WT-FLAG
Plasmid#199679PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED-FlagDepositorInsertLeucine zipper LYCHOS LED WT (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine
Plasmid#228902PurposeFor AAV packaging and Cre-dependent expression of a peripherally restricted DREADDDepositorInserthydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
UseAAV and Synthetic BiologyTagsHA and P2A mCitrineExpressionMammalianMutationR108KPromoterChicken Beta ActinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC52A2
Plasmid#131902PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC52A2 (GPR172A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC52A2_STOP
Plasmid#161068PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC52A2 (GPR172A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC52A1_STOP
Plasmid#161335PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC52A1 (SLC52A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO shLYCHOS/LYCHOS WT-FLAG
Plasmid#209315PurposeLentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)DepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/FLAG Metap2
Plasmid#209314PurposeLentiviral construct expressing FLAG Metap2 and shRNA against LYCHOSDepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS FP>IA-HA-GFP
Plasmid#199688PurposeExpression of LYCHOS FP>IA mutant in insect cellsDepositorInsertLYCHOS (GPR155 Human)
UseTags10xHis, 6xHis, EGFP, HA, and HRV 3CExpressionInsectMutationF43 and P44 mutated to I and A respectivelyPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED 4CA-FLAG
Plasmid#199681PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-FlagDepositorInsertLeucine zipper LYCHOS LED 4CA (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationC595.604.629.638 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED Y551A-FLAG
Plasmid#199680PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-FlagDepositorInsertLeucine zipper LYCHOS LED Y551A (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationY551 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-OXER1-mKate2
Plasmid#226713Purposeoverexpression red fluorescent OXER1 protein in zebrafish larvaeDepositorInsertOXER1 (OXER1 Human)
UseUnspecified; Zebrafish expressionTagsmKate2ExpressionMutationPromoterSP6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-BCL2 3'UTR
Plasmid#84597PurposeRenilla luciferase reporter containing BCL2 3' UTRDepositorInsertBCL2 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-TOB1 3'UTR
Plasmid#84589PurposeRenilla luciferase reporter containing TOB1 3' UTRDepositorInsertTOB1 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIVEX2.3 TEM-5
Plasmid#16561DepositorInsertTEM5 (ADGRA2 Human)
UseTags6xHisExpressionBacterialMutationExtracellular regionPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE4 CTF-FLAG
Plasmid#217692PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE4 (ADGRE4P Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-173PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only