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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,670 results for: nif

Showing: 181 - 200 of 1670 results

  1. pGBT9-FHIP

    Plasmid
    #198527
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979
    Insert
    FHIP (FHIP1B Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent mutations in codons 353 and 651 as well as…
    Promoter
    ADH1
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198527-plasmid-map-sequence-id-393106

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  2. pGBT9-Hook1

    Plasmid
    #198523
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979
    Insert
    Hook1 (HOOK1 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent substitution in codon 620 to eliminate int…
    Promoter
    ADH1
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198523-plasmid-map-sequence-id-389375

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  4. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  7. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    P45
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  8. pKS1531

    Plasmid
    #239521
    Purpose
    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239521-plasmid-map-sequence-id-475896

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  15. pAAV-CAG-RLuc

    Plasmid
    #83282
    Purpose
    recombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)
    Depositor
    Mark Kay
    Insert
    Renilla Luciferase
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    83282-plasmid-map-sequence-id-158421

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Showing: 181 - 200 of 1670 results