We narrowed to 3,906 results for: SPL;

1. pMSCV-IRES-GFP-MyD88-NpLxIS

   Plasmid

   #131347

   Purpose

   Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   MyD88-NpLxIS (Myd88 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSecEffector

   Plasmid

   #172891

   Purpose

   Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   Bxb1
   TP901
   TEV Display cassette
   ProD promoter between anti aligned Bxb1 sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProD, nTETO, and pBAD /AraC

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS416-Aga1p-FAPB2.3.6

   Plasmid

   #158147

   Purpose

   Yeast display reporter for non-display yeast strains (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBG-TFII-I beta

   Plasmid

   #22155

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pEBB-II-I-EGFP delta isoform

   Plasmid

   #22149

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_(GLuc)_TOP1

   Plasmid

   #68423

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH-PanCen TALE-mVenusN

   Plasmid

   #191395

   Purpose

   TALE fused to N-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   N-terminal part of split mVenus
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 7iBi89 (rat fibronectin)

   Plasmid

   #14065

   Depositor

   Richard Hynes

   Article

   Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

   Insert

   Rat Fibronectin 7iBi89 (Fn1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Insert was cut out of pGEM3 with SalI and SacI an…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pTRCHisB-TRF2∆B

   Plasmid

   #53208

   Purpose

   Expressed N-terminally Hexahistine tagged TRF2 Delta Basic protein

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 Delta Basic (TERF2 Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   contains amino acids 47-500

   Promoter

   Trc

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-IRES-GFP-MyD88-CpLxIS

    Plasmid

    #131348

    Purpose

    Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its C-terminus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88-CpLxIS (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 FLIPE-10u

    Plasmid

    #13542

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207R in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

    Plasmid

    #168189

    Purpose

    HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-pEF1a-rtTAc

    Plasmid

    #172121

    Purpose

    Constitutive expression of InteinC-rtTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-rtTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB

    Plasmid

    #131350

    Purpose

    Expresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase B

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88-GyrB (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Flag-Tpr-Si

    Plasmid

    #60918

    Purpose

    Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    TPR (TPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations in siRNA target region; E1…

    Promoter

    CMV

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3_4xUAS_CP-candidate_luc+

    Plasmid

    #125154

    Purpose

    STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-His-Flag-Tipin

    Plasmid

    #22890

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    Flag- and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pET:GCAT:GFP

    Plasmid

    #60736

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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