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Plasmid#45521DepositorInsertPKA catalytic subunit alpha tagged by mEGFP
ExpressionMammalianAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJ3H-Mst2 K56R
Plasmid#12206DepositorAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Puro CAG FUCCI
Plasmid#136934Purposedonor plasmid for constitutive FUCCI expression in human cellsDepositorInsertClover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
UseSynthetic BiologyTagsClover and mKO2ExpressionMammalianPromoterCAGAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorPublicationInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
TagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…Available sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
TagsMitochondrial targeting signalExpressionMammalianPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-eGFP-LIC
Plasmid#40768DepositorTypeEmpty backboneTagseGFPExpressionMammalianPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A
Plasmid#100146PurposeExpresses CPT1ADepositorAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECE-HA-AMPKbeta1 wt
Plasmid#31666DepositorInsertprotein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)
TagsHA (YDVPDYASL)ExpressionMammalianAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFPC1-EPSIN 1
Plasmid#22228DepositorPublicationInsertEpsin 1 (Epn1 Rat)
TagsGFPExpressionBacterial and MammalianMutationplease see depositor comment belowAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV1)
Viral prep#162382-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINJ
Plasmid#26045DepositorPublicationInsertHisGST3C-lacZ
TagsMAHHHHHH_GST_LEVLFQGTExpressionBacterial, Insect, and Mamm…Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
TagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
TagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-GroEL/ES
Plasmid#83922PurposeCo-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.DepositorInsertsSkp
Gro EL/ES
ExpressionBacterialPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SomaGCaMP6f2 (AAV9)
Viral prep#158757-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG promoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc mut MBS1-4
Plasmid#16565DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant MBS1-4Available sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 IKKE K38A flag
Plasmid#26202DepositorInsertI-kappa-B kinase epsilon K38A (IKBKE Human)
TagsFlagExpressionMammalianMutationCatalytic Lysine changed to Alanine (K38A)Available sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by