We narrowed to 2,799 results for: fas

1. pRDA355_sgCiPELO #2

   Plasmid

   #229020

   Purpose

   Expression of a CRISPRi doxycycline inducible guide targeting PELO

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Insert

   PELO gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. nLuc_AP4_TEVs_P3mS

   Plasmid

   #119299

   Purpose

   Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
   

   Use

   Luciferase

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AP4mS_PPVs_P3_cLuc

   Plasmid

   #119300

   Purpose

   Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Mito-mGreenLantern

   Plasmid

   #164461

   Purpose

   Localization of mGreenLantern fluorescent protein to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-Laconic/pCMV-myc-nuc

   Plasmid

   #46307

   Purpose

   a nuclear-targeted, genetically-encoded sensor for lactate

   Depositor

   Luis Felipe Barros

   Article

   Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

   Insert

   NLS-Laconic
   

   Use

   Lactate biosensor

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSN068

   Plasmid

   #101749

   Purpose

   Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available Since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. H2B-mGreenLantern

   Plasmid

   #164464

   Purpose

   Localization of mGreenLantern fluorescent protein to histone 2B

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Tags

   Human Histone 2B (D26G and V119I mutations)

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-mGreenLantern

   Plasmid

   #164469

   Purpose

   Constitutive CAG-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CAG

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

   Plasmid

   #113899

   Purpose

   Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   TetR-HA-NLS-P2A-BSD-Myc
   

   Use

   Lentiviral

   Tags

   HA-Myc

   Promoter

   CMV-MIE

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. LifeAct-mGreenLantern

    Plasmid

    #164459

    Purpose

    Localization of mGreenLantern fluorescent protein to actin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-dLight1.3b

    Plasmid

    #125560

    Purpose

    For expression of the genetically encoded dopamine sensor dLight1.3b

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Clathrin-mGreenLantern

    Plasmid

    #164462

    Purpose

    Localization of mGreenLantern fluorescent protein to clathrin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Clathrin

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

    Plasmid

    #113898

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-dLight1.1

    Plasmid

    #111067

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1 and AAV5

    Insert

    dLight1.1
    

    Use

    AAV

    Tags

    Flag tag

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pCSN067

    Plasmid

    #101748

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGY577

    Plasmid

    #166663

    Purpose

    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    Cre: E340A, D341A

    Promoter

    CMV

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

    Plasmid

    #158585

    Purpose

    Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    membrane localization signal-tagBFP-wrmScarlet11
    

    Tags

    tagBFP

    Expression

    Yeast

    Mutation

    GGGS linker

    Promoter

    TEF

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+8 Flag3HuR

    Plasmid

    #135950

    Purpose

    Expresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Tags

    Flag3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. ER-mGreenLantern

    Plasmid

    #164463

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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