We narrowed to 6,508 results for: nls
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Plasmid#220745PurposeNLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyTagsNLS-VP16ExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nDHFR173-gs10-NLS-VPR
Plasmid#188262PurposePlasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnDHFR173 (DHFR Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
nGR2175-gs10-NLS-VPR
Plasmid#188264PurposePlasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnGR2175
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS
Plasmid#145268PurposeMammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLSDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSSM97: pCMV-HA-NLS-SpuFz1-E498R
Plasmid#205275PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498RDepositorInsertSpuFz1
TagsHA, NLS(Makkerh)ExpressionMammalianMutationE498RAvailable SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM105: pCMV-HA-NLS-SpuFz1-T513K
Plasmid#205276PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513KDepositorInsertSpuFz1
TagsHA, NLS(Makkerh)ExpressionMammalianMutationT513KAvailable SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM58: pCMV-HA-NLS-SpuFz1-D487K
Plasmid#205274PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487KDepositorInsertSpuFz1
TagsHA, NLS(Makkerh)ExpressionMammalianMutationD487KAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM59: pCMV-HA-NLS-SpuFz1-D300R
Plasmid#205272PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300RDepositorInsertSpuFz1
TagsHA, NLS(Makkerh)ExpressionMammalianMutationD300RAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-All-in-one-hCascade
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
ExpressionMammalianPromoterCAGAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)
Plasmid#114074PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationS542R, K548V and N552RAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A
Plasmid#127660PurposeOver-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227ADepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-C–SaKKH-Cas9(D10A)
Plasmid#157840Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseCRISPRAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107318PurposeExpresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107320PurposeExpresses SpCas9 fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to SaCas9PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-nlsBFP
Plasmid#169029PurposegRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1cNLSdN243-C1
Plasmid#131848PurposeExpresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
TagsHcRedExpressionMammalianMutationThis is a deletion contruct of the cytoplasmic m…Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Catalytically Enhanced (E655G and N696I) -ThermoCas9-3xNLS
Plasmid#254684PurposeExpresses Catalytically Enhanced (E655G and N696I) ThermoCas9 with 3x NLS tags. Utilized for RNP transfectionDepositorInsertCatalytically Enhanced (E655G and N696I) ThermoCas9
TagsNLS, SV40 NLS, and nucleoplasmin NLSExpressionBacterialAvailable SinceMay 6, 2026AvailabilityAcademic Institutions and Nonprofits only