We narrowed to 15,156 results for: pET

1. pET19b+H2B(T116C)

   Plasmid

   #157808

   Purpose

   Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H2B(T116C)
   

   Expression

   Bacterial

   Mutation

   T116C

   Promoter

   T7

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b(+)_MBP-nLucCP-67/68

   Plasmid

   #113922

   Purpose

   Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   MBP-NanoLucCP-67/68
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET151-hUCH37 (ISF1)

   Plasmid

   #61929

   Purpose

   Expression of hUCH37 (ISF1) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) (UCHL5 Human)
   

   Tags

   6x HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-SpyCatcher002-MBP

   Plasmid

   #216295

   Purpose

   Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher002-MBP
   

   Tags

   His6 tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-51b(+)-Caprola_13

   Plasmid

   #194674

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_13
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24-ISWI-iCBD

   Plasmid

   #105499

   Purpose

   Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

   Depositor

   James Kadonaga

   Article

   Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

   Insert

   ISWI-iCBD
   

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-His-MinD_MinE

   Plasmid

   #133621

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

   Depositor

   Petra Schwille

   Article

   Loose et al Science. 2008 May 9;320(5877):789-92. do

   Insert

   His-MinD_MinE (minD, minE E. coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-51b(+)-Caprola_01

   Plasmid

   #194662

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30-T7-His-sfGFP

    Plasmid

    #180755

    Purpose

    pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Tags

    strep tag

    Expression

    Bacterial

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. wt 6XHis-SNX9 pET15b

    Plasmid

    #34690

    Depositor

    Sandra Schmid

    Article

    Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

    Insert

    Sorting nexin 9
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pETDuet-1 Flag-LplA

    Plasmid

    #232118

    Purpose

    For bacterial expression of Flag-tagged E. coli LplA.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    lplA (lplA Escherichia coli (strain K12))
    

    Tags

    Flag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-hAIM2-His6

    Plasmid

    #51532

    Purpose

    Express His6-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Tags

    His6 and T7

    Expression

    Bacterial

    Promoter

    lac operator

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Expression

    Bacterial

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-51b(+)-cpHalo∆

    Plasmid

    #194659

    Purpose

    Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHalo∆
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-HRASLS3-1-132

    Plasmid

    #100725

    Purpose

    Expression of N-terminal region of human HRASLS3 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    HRASLS3 (PLAAT3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-AqpZ-ALFA

    Plasmid

    #212310

    Purpose

    Expression of AqpZ with ALFA tag

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    Aquaporin Z
    

    Tags

    ALFA tag and Strep-Tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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