We narrowed to 11,352 results for: SET

1. pT040

   Plasmid

   #67640

   Purpose

   URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Insert

   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS1021

   Plasmid

   #59575

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
   

   Use

   E. coli cloning vector

   Tags

   Twin-Strep tag

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b-sfGO-Matryoshka-LE-LP

   Plasmid

   #100018

   Purpose

   Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   cpsfGFP-LSSmOrange
   

   Use

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Leu and Glu N-term linker and Leu and Pro C-term …

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b-eGO-Matryoshka-LE-LP

   Plasmid

   #100017

   Purpose

   Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   cpEGFP-LSSmOrange
   

   Use

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Leu and Glu N-term linker and Leu and Pro C-term …

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYES2-gRNA-hyg-MCS

   Plasmid

   #107734

   Purpose

   E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   X-3 site gRNA expression cassettes
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52p

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGS1022

   Plasmid

   #59576

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
   

   Use

   E. coli cloning vector

   Tags

   Twin-Strep tag

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGS1212

   Plasmid

   #63785

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eGFP-Strep-tag II followed by URA3 selection marker
   

   Use

   E. coli cloning vector

   Tags

   eGFP-Strep-tag II

   Expression

   Mutation

   Promoter

   none

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. KG#797

   Plasmid

   #110916

   Purpose

   Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   unc-129 promoter
   STRN-1 (Sentryn)-GFP
   unc-54 3' control region with 1 intron just upstream and 1 intron in control region
   

   Use

   Tags

   GFP (contains 3 artificial introns)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGS1613

   Plasmid

   #63789

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   tDimer2-Strep-tag II followed by HIS5
   

   Use

   E. coli cloning vector

   Tags

   tDimer2-Strep-tag II

   Expression

   Mutation

   Promoter

   none

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS1122

    Plasmid

    #63790

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eSapphire-Twin-Strep tag followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    eSapphire-Twin-Strep tag

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGS1412

    Plasmid

    #63787

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eCitrine-Strep-tag II followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    eCitrine-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLTR-X-GFP

    Plasmid

    #58245

    Purpose

    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP

    Depositor

    Stephan Geley

    Article

    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

    Inserts

    attR1-Cm(R)-CcdB-attR2
    TetR-NLS-T2A-GFP
    

    Use

    Lentiviral and RNAi; Gateway destionation vector

    Tags

    GFP, NLS, T2A, and TetR

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and no

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid

    #67275

    Purpose

    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

    Insert

    puromycin resistant gene
    

    Use

    Tags

    linked to red flouresent protein gene (katushka)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc-y1sgRNA-Cas9

    Plasmid

    #49331

    Purpose

    Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    y1 sgRNA (y Fly)
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR P2R-P3-V5-6xHis

    Plasmid

    #48347

    Purpose

    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    V5 and 6xHis epitope tags cassette
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Contains a stop codon at the end of the epitope t…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGLTR-S-PURO

    Plasmid

    #58244

    Purpose

    GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance

    Depositor

    Stephan Geley

    Article

    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

    Inserts

    attR1-Cm(R)-CcdB-attR2
    Puro(R)
    

    Use

    Lentiviral and RNAi; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and no

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NEK7_HUMAN_D0

    Plasmid

    #79735

    Purpose

    This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK7 (NEK7 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. VRK1_HUMAN_D0

    Plasmid

    #79684

    Purpose

    This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. KCC2B_HUMAN_D0

    Plasmid

    #79718

    Purpose

    This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2B
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only