We narrowed to 31,136 results for: ica

1. pGS_N_Strep

   Plasmid

   #101651

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS_C_Strep

   Plasmid

   #101652

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLhT-pHG165a V52A

   Plasmid

   #90050

   Purpose

   Constitutive expression of a chimeric LacI:TreR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhT/V52A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V52A

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLhA-pHG165a Q55A

   Plasmid

   #90052

   Purpose

   Constitutive expression of a chimeric LacI:AscG transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhA/Q55A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q55A

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLhC-pHG165a Q55I

   Plasmid

   #90053

   Purpose

   Constitutive expression of a chimeric LacI:CytR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhC/Q55I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q55I

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CC1

   Plasmid

   #63713

   Purpose

   Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

   Depositor

   Bradley Baker

   Article

   Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

   Insert

   CC1
   

   Use

   Tags

   Super ecliptic pHlorin A227D

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA4-rB90

   Plasmid

   #18959

   Depositor

   Mariano Viapiano

   Article

   Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

   Insert

   Brevican C-terminus (Bcan Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Rat brevican C-terminus: Signal peptide aa 1-22 …

   Promoter

   Available since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

8. DCT.VV.Orf46

   Plasmid

   #12734

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf46
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pRK5-HA-Ubiquitin-K48R

   Plasmid

   #17604

   Purpose

   Mammalian expression of HA tagged ubiquitin K48R

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48R (expected to disrupt G76-K48 ubiquitin chain…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-NlaCascade-NLS-6xHis

    Plasmid

    #180213

    Purpose

    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas5-Cas8-Cas7
    

    Use

    Tags

    6XHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-CMV-jGCaMP8f

    Plasmid

    #162373

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Aequorea victoria, Mouse)
    

    Use

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV SYN PSAM4 GlyR IRES EGFP

    Plasmid

    #119742

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-jGCaMP8m

    Plasmid

    #162372

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLViP-LifeAct-iRFP670

    Plasmid

    #229699

    Purpose

    Lentiviral expression of LifeAct-iRFP670 in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

    Plasmid

    #130996

    Purpose

    Double floxed ChRmine-eYFP under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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