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pEM705 Gsi (Cys)

Plasmid

#109373

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAM420 GFP-titin-ssrA substrate

Plasmid

#226362

Purpose

Expression of GFP-titin-ssrA substrate for 26S proteasome

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

GFP-titin substrate

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-TurboID-EGFP

Plasmid

#163846

Purpose

In vitro transcription of TurboID-EGFP. Parton lab clone JRS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-lyn10-mCherry-dLOVPLD

Plasmid

#219556

Purpose

PM-targeted catalytically dead LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-mCherry-dLOVPLD

Tags

mCherry

Expression

Mammalian

Mutation

H170A mutation on PLD

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-p18-mCherry-LOVPLD

Plasmid

#219560

Purpose

lysosome-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

p18-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pREDusk-AmpR-MCS

Plasmid

#188974

Purpose

For red light-repressed bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAG1171 FIREmate-ECFP-Giantin

Plasmid

#205166

Purpose

Expresses FIREmate-ECFP-Giantin in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Giantin

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-IVT

Plasmid

#215836

Purpose

Template for in vitro transcription of CBE6d (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAG424-10X-tRNA

Plasmid

#70125

Purpose

Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

Depositor

Jay Hesselberth

Article

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Expression

Yeast

Promoter

None – SUP4 pol III promoter provided by insert

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
U_(pos36GFP)-Cys

Plasmid

#71758

Purpose

U_+36GFP-Cys fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_+36GFP-Cys

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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