163,297 results

1. pET30-HPF1-His-Sumo-Flag

   Plasmid

   #111577

   Purpose

   Expresses full length HPF1 in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   HPF1 (HPF1 Human)
   

   Use

   Tags

   6xHis-Sumo-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

   Plasmid

   #189807

   Purpose

   Retroviral delivery of Cas9

   Depositor

   Ellen Rothenberg

   Article

   Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

   Insert

   Cas9
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNCS mCherry

   Plasmid

   #91769

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Cherry
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   T7

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1464

   Plasmid

   #144104

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   VAV1 (VAV1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV Flag PAI-RBP

   Plasmid

   #45452

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SV40-MCS-Z1

   Plasmid

   #127639

   Purpose

   Empty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistance

   Depositor

   Jordan Green

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 promoter

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bassik lab Mouse CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases

   Pooled Library

   #1000000122

   Purpose

   CRISPR gRNA mouse knockout pooled library - Drug targets, kinases, phosphatases

   Depositor

   Michael Bassik

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-PL

   Plasmid

   #20783

   Depositor

   Bryan Cullen

   Article

   Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

10. Prab-3::NLS::GFP

    Plasmid

    #68120

    Purpose

    Nuclear Localized GFP for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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