We narrowed to 152,634 results for: des

1. CMV-OMM-GCaMP6f

   Plasmid

   #127874

   Purpose

   Fluorescent reporter for mitochondrial outer membrane Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Outer membrane localized GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDRF1-GW ymNeongreen-T2A-mCherry

   Plasmid

   #125700

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDRF1-GW ymScarletI-T2A-mTurquoise2

   Plasmid

   #118441

   Purpose

   Produces equimolar expression of yeast codon-optimized mScarletI and mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymScarletI-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV4-BlaM-Vpr

   Plasmid

   #21950

   Depositor

   Warner Greene

   Article

   Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

   Insert

   BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

5. PBAD-his6-prkA-pACYC184

   Plasmid

   #41041

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   prkA
   

   Use

   Tags

   6xHis and T7 epitope tag

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV_ADRB2-mTurquoise2-iLID

   Plasmid

   #161002

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   ADRB2-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

   Plasmid

   #32703

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Cre/Lox and Retroviral

   Tags

   3x HA tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRN114

   Plasmid

   #127221

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, Wus, AtSTM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSA293-CHEQ-seq

   Plasmid

   #174669

   Purpose

   Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.

   Depositor

   Stein Aerts

   Article

   Mauduit et al Elife. 2021 Dec 7;10. pii: 71735. doi: 1

   Type

   Empty backbone

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   SCP1

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMKV059

    Plasmid

    #133314

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+, IPT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUbi10, 35S

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFGAMS-EGFP

    Plasmid

    #99107

    Purpose

    Expresses human PFAS-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PFAS (PFAS Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MS2_GFP

    Plasmid

    #61764

    Purpose

    Expresses MS2 coat protein fused to GFP

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    MS2 coat protein fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCTCON2-GFP

    Plasmid

    #158130

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPPAT-EGFP

    Plasmid

    #99105

    Purpose

    Expresses human PPAT-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMKV058

    Plasmid

    #133313

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+. WUS2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUbi10, Nos

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PP7_mCherry

    Plasmid

    #61763

    Purpose

    Expresses PP7 coat protein fused to mCherry

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    PP7 coat protein fused to mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFLAG-HHARI

    Plasmid

    #17450

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    HHARI (ARIH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUF-Cas9-pre-sgRNA

    Plasmid

    #137845

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MCP-EGFP-PiggyBac

    Plasmid

    #190003

    Purpose

    Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

    Depositor

    Tineke Lenstra

    Article

    Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

    Insert

    MCP-EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEX128·gRNA

    Plasmid

    #187600

    Purpose

    Template for amplification of gRNA with Cas6 recognition site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    gRNA template
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    no promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSGV Hu Acceptor

    Plasmid

    #64269

    Purpose

    Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pIVEX-SNAP-GFP

    Plasmid

    #46846

    Purpose

    SNAP-GFP fusion

    Depositor

    Florian Hollfelder

    Article

    Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

    Insert

    SNAP-GFP fusion
    

    Use

    In vitro expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDRF1-GW yoeGFP-T2A-mCherry

    Plasmid

    #118446

    Purpose

    Produces equimolar expression of yeast codon-optimized eGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yoeGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pREDusk-AmpR-MCS

    Plasmid

    #188974

    Purpose

    For red light-repressed bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRF2-IRESS-eGFP

    Plasmid

    #19798

    Depositor

    Mark Mattson

    Article

    van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

    Insert

    TRF2 (TERF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    When compared to GenBank reference sequence NP_00…

    Promoter

    CMV-F

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

27. px458_Conc2

    Plasmid

    #134449

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMOD_C'5014

    Plasmid

    #127219

    Purpose

    Module C' with Pnos::WUS2

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px458_Conc3

    Plasmid

    #134450

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. rbcLS-pMAL-2px

    Plasmid

    #41621

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    rbcL/rbcS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCTCON2-BXG

    Plasmid

    #158127

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    mTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDRF1-GW yosfGFP-T2A-mCherry

    Plasmid

    #118448

    Purpose

    Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yosfGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVENUS-N1-POLR2G-GFP

    Plasmid

    #86872

    Purpose

    To express POLR2G (RPB7) tagged with VENUS at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    POLR2G (POLR2G Human)
    

    Use

    Tags

    VENUS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCTCON2-BYG

    Plasmid

    #158126

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    mTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-FAK W266A

    Plasmid

    #186149

    Purpose

    For expressing FAK in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPROK AdoHcyase

    Plasmid

    #14683

    Depositor

    Michael Hershfield

    Article

    Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

    Insert

    adenosyl-homocysteine hydrolase (AHCY Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

37. 131-2a_LC

    Plasmid

    #215331

    Purpose

    Expression monoclonal antibody 131-2a LC

    Depositor

    Joost Snijder

    Article

    Peng et al bioRxiv 2025.01.04.631317

    Insert

    131-2a_LC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 131-2a_HC

    Plasmid

    #214965

    Purpose

    Expression monoclonal antibody 131-2a

    Depositor

    Joost Snijder

    Article

    Peng et al bioRxiv 2025.01.04.631317

    Insert

    131-2a_HC
    

    Use

    Tags

    his8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS306-PTDH3-SUMO10-6SIM

    Plasmid

    #190248

    Purpose

    Expression SUMO10-SIM6 in yeast cells

    Depositor

    Liam Holt

    Article

    Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

    Insert

    10SUMO-6SIM
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pREDawn-StrR-MCS

    Plasmid

    #188979

    Purpose

    For red light-induced bacterial expression of gene of interest (StrR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge