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pDEST-ORF-mScarlet

Plasmid

#217453

Purpose

Gateway destination vector with C-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Type

Empty backbone

Use

Gateway destination vector

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-mScarlet-ORF

Plasmid

#217454

Purpose

Gateway destination vector with N-terminal mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Type

Empty backbone

Use

Gateway destination vector

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-YPet

Plasmid

#215540

Purpose

Gateway entry vector with the YPet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

YPet

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Hv_HPT:pGC1:Gus_introns

Plasmid

#215195

Purpose

Evaluating guard cell specific promotors in Barley

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

GA-stimulated regulatory protein

Use

Synthetic Biology

Expression

Plant

Promoter

AtGC1

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bo_NPTII:pGC1:Gus_introns

Plasmid

#215181

Purpose

Evaluating guard cell specific promotors in Brassica

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

GA-stimulated regulatory protein

Use

Synthetic Biology

Promoter

AtGC1

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-tetR

Plasmid

#238043

Purpose

Modified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

ttrB

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc100_CSC

Plasmid

#210592

Purpose

Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_Opt_Renilla_CSC

Plasmid

#210593

Purpose

Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_OptλHAramp-FLuc100

Plasmid

#210577

Purpose

Expresses Optimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal lambda HA-Optimal Firefly luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_NonoptλHAramp-FLuc100

Plasmid

#210579

Purpose

Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Nonoptimal lambda HA-Optimal Firefly luciferase

Use

Lentiviral and Luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-pA

Plasmid

#210584

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc0_CSC

Plasmid

#210589

Purpose

Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)

Use

Luciferase

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM2

Plasmid

#225289

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC311 for pJBM2

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Ub-LPNTG-pET

Plasmid

#221850

Purpose

Expresses Ub with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Ubiquitin

Expression

Bacterial

Mutation

Arg74ProLeuThr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine

Plasmid

#197681

Purpose

"Insert(high)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine

Plasmid

#197683

Purpose

"Insert(low)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM142_phiC31-nTRE-pEF1s(high)(CC793)-H2B-citrine

Plasmid

#197685

Purpose

"pEF1s(high)" reporter plasmid, with CpG793 mutated to CC

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4E-I96AI112A-siRNAres_Z

Plasmid

#148163

Purpose

Insect Expression of DmeIF4E-I96AI112A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E-I96AI112A

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4E-W106A-siRNAres_Z

Plasmid

#148164

Purpose

Insect Expression of DmeIF4F-W106A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4F-W106A

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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