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We narrowed to 7,175 results for: ef1a

Showing: 2041 - 2060 of 7175 results

  1. ELL1 CtoA

    Plasmid
    #127279
    Purpose
    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHNK by Q5 mutagenesis
    Promoter
    EF1a
    Available since
    Nov. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    127279-plasmid-map-sequence-id-249989

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  2. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  3. pKAM-E-Flag-BFP

    Plasmid
    #101867
    Purpose
    Lentiviral Evi-1-Flag with dual BFP and puro markers
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101867-plasmid-map-sequence-id-211116

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  4. pKAM-ME-Flag-BFP

    Plasmid
    #101868
    Purpose
    Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers
    Depositor
    Archibald Perkins
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101868-plasmid-map-sequence-id-211131

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  5. MLCK_pLVX-EF1-Cry2-mCherry

    Plasmid
    #64369
    Purpose
    lentiviral expression of Myosin Light Chain Kinase and Cry2-mCherry
    Depositor
    Matthieu Piel
    Insert
    Myosin Light Chain Kinase (MYLK Human)
    Use
    Lentiviral
    Tags
    Cry2-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. PB-TO-MYOD1-shOct4

    Plasmid
    #182309
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    shOct4
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182309-plasmid-map-sequence-id-359523

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  9. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  10. AAV-hACE2-cMYC-Flag

    Plasmid
    #158957
    Purpose
    Used for expression of human ACE2 with AAV packaging
    Depositor
    Akiko Iwasaki
    Insert
    Human ACE2 (ACE2 Human)
    Use
    AAV
    Tags
    Flag and MYC
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    158957-plasmid-map-sequence-id-310391

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  12. pEF1-puro/hpb1

    Plasmid
    #59976
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 headpiece (ITGB1 Human)
    Use
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a promoter
    Available since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pEF1-puro/hpb1(short)

    Plasmid
    #59973
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 (short) (ITGB1 Human)
    Use
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a promoter
    Available since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2041 - 2060 of 7175 results