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pEBG-TFII-I Y248&249F

Plasmid

#22189

Depositor

Ananda Roy

Article

Novina et al J Biol Chem. 1998 Dec 11. 273(50):33443-

Insert

TFII-I (GTF2I Human)

Tags

GST

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines.

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pM2_PLLacO1_R1_mTagBFP2

Plasmid

#250898

Purpose

mTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mTagBFP2

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux*_R1_EGFP

Plasmid

#250901

Purpose

EGFP expressed under PLux* promoter; part of BNeu AS14; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Mutation

PLux* = Point mutation in PLux

Promoter

PLux*

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux*_R1_mKO2

Plasmid

#250902

Purpose

mKO2 expressed under PLux* promoter; component of BNeu AS14*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mKO2

Use

Synthetic Biology

Mutation

PLux* = Point mutation in PLux

Promoter

PLux*

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PJ23102R1LuxR_PCTR1E2Crimson

Plasmid

#250892

Purpose

LuxR expressed under BBa_J23102; E2-Crimson under PCT synthetic promoter; component of BNeuAS6; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

E2-Crimson
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLTetO1_R1_EGFP

Plasmid

#250899

Purpose

EGFP expressed under PL TetO1 promoter; part of BNeu AS12; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL TetO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PCTR1EGFP_PJ23102R1LuxR

Plasmid

#250885

Purpose

EGFP expressed under PCT synthetic promoter and LuxR expressed under BBa_J23102 promoter; component of BNeuAS3; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

EGFP
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHLT_R1_E2Crimson

Plasmid

#250886

Purpose

E2-Crimson expressed under PHLT synthetic promoter; component of BNeuAS4; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PHLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_E2Crimson

Plasmid

#250882

Purpose

E2-Crimson expression under PLT synthetic promoter; part of BNeu AS1*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PR_R1_E2Crimson

Plasmid

#250896

Purpose

E2-Crimson expressed under PR promoter; component of Bneu AS10; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PR

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHT_R4_EGFP

Plasmid

#250889

Purpose

EGFP expressed under PHT synthetic promoter and regulated by RBS 'R4'; part of BNeuAS9; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-MS2-CP-GFP-SBP

Plasmid

#249048

Purpose

Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP)

Depositor

Jeffrey Gerst

Article

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Insert

MS2 binding coat protein

Tags

GFP and streptavidin binding peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet-H2B

Plasmid

#244034

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-HIST1H2BJ

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet-CAAX

Plasmid

#244033

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to plasma membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet-CAAX

Plasmid

#244032

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to plasma membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet-H2B

Plasmid

#244035

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-HIST1H2BJ

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6401

Plasmid

#183838

Purpose

sfGFP reporter under the control of WT E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2-3A

Plasmid

#238984

Purpose

In vitro transcription of mutant bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Celver et al J Biol Chem. 2002 Mar 15;277(11):9043-8.

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

I386A,V387A,F388A

Promoter

SP6

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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