Addgene

We narrowed to 7,019 results for: 11

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pWZ414-F15

Plasmid

#23064

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 deleted amino acids 4 - 19

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F23

Plasmid

#23065

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Glutamine

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F33

Plasmid

#23068

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Glutamine H4 K5Q

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F47

Plasmid

#23071

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysines 8 and 16 to Glutamines…

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F49

Plasmid

#23073

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysines 8 and 16 to Arginine, …

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F51

Plasmid

#23075

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysines 5 and 12 to Glutamine,…

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F52

Plasmid

#23076

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysines 5 and 12 to Arginines,…

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F36

Plasmid

#23069

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glycine, H3 K14G

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F43

Plasmid

#23070

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
p031 pCS2 End-1 ORF

Plasmid

#11030

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End-1 (end-1 Nematode)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pEYmRD3A

Plasmid

#127756

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach,RFP Divergent Orientation

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-PPO-Venus

Plasmid

#139505

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV8 and AAV9

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-PPO-Venus

Plasmid

#139504

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV5

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2

Plasmid

#52961

Purpose

Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 WT

Plasmid

#16233

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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SIRT1 Flag

Plasmid

#13812

Purpose

Mammalian expression of human SIRT1 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT1 (SIRT1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

N-terminus truncated

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pMSC234

Plasmid

#216357

Purpose

xhNup93-Nb3t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup93-Nb3t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC168

Plasmid

#216350

Purpose

xhNup35-Nb1t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup35-Nb1t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge