We narrowed to 7,175 results for: ef1a

1. CROPseq-Guide-Zeo

   Plasmid

   #127173

   Purpose

   Entry plasmid for guide cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-RB

   Plasmid

   #60522

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-dCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201152

   Purpose

   Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   dCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: TACGTTCTCTATCACTGAT

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi-Neo

   Plasmid

   #60525

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ11438

   Plasmid

   #183204

   Purpose

   pHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPRE

   Depositor

   Stanley Qi

   Article

   Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

   Insert

   hyperdCas12 base editor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBbi-RH

   Plasmid

   #60516

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSBbi-BB

   Plasmid

   #60521

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSBbi-RN

   Plasmid

   #60519

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSBbi-BP

   Plasmid

   #60512

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBbi-GH

    Plasmid

    #60514

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSBbi-BH

    Plasmid

    #60515

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBbi-BN

    Plasmid

    #60518

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-AP.Adamts2.1-GFP-WPRE

    Plasmid

    #211488

    Purpose

    expresses GFP under artificial promoter Adamts2

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAF124

    Plasmid

    #222372

    Purpose

    Recombinant human IgG1 antibody (3D5) that binds to 6xHis at the C-terminus of proteins

    Depositor

    Andrew S. Flies

    Article

    Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

    Insert

    IGHG
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKER-mKOk

    Plasmid

    #85975

    Purpose

    Expresses mKOk from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mKOk
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKER-mRuby2

    Plasmid

    #85977

    Purpose

    Expresses mRuby2 from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKER-mCardinal

    Plasmid

    #85976

    Purpose

    Expresses mCardinal from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGH916

    Plasmid

    #230842

    Purpose

    Ef1a-DivA-BE3-T2A-tagBFP-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

    Insert

    DivA-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGH912

    Plasmid

    #230855

    Purpose

    Ef1a-DivA-BE3-T2A-BlastR-wPRE

    Depositor

    Gaelen Hess

    Article

    Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

    Insert

    DivA-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CROPseq-3P5P

    Plasmid

    #219680

    Purpose

    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer

    Depositor

    Christoph Bock

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge