Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,479 results for: puc19

Clear keywords
Showing: 2081 - 2100 of 2479 results

  6. pYI2206

    Plasmid
    #228348
    Purpose
    Constitutive rPhlTA mutant expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228348-plasmid-map-sequence-id-457096

    Enlarge

  14. qTAG-C-Solo-miRFP670nano3-H2BC11

    Plasmid
    #227331
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227331-plasmid-map-sequence-id-452128

    Enlarge

  15. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

    Enlarge

  17. pToxEXPr6

    Plasmid
    #218290
    Purpose
    Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218290-plasmid-map-sequence-id-433735

    Enlarge

  18. pJQR3

    Plasmid
    #218289
    Purpose
    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218289-plasmid-map-sequence-id-433691

    Enlarge

  19. pITEdv1

    Plasmid
    #218283
    Purpose
    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218283-plasmid-map-sequence-id-433685

    Enlarge

  20. pITEu2

    Plasmid
    #218282
    Purpose
    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218282-plasmid-map-sequence-id-433684

    Enlarge

Showing: 2081 - 2100 of 2479 results