We narrowed to 28,498 results for: Tec

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eA3A-BE3 (pRZ206)

Plasmid

#131315

Purpose

CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-IL35-IRES-mCherry-PGK-BFP

Plasmid

#223210

Purpose

Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

IL35 (IL12A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP

Plasmid

#223208

Purpose

Lentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

PDL1 (CD274 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-xBE3-P2A-Puro

Plasmid

#110870

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE3

Use

Lentiviral

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

Plasmid

#208960

Purpose

A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

Plasmid

#114087

Purpose

U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for FnCas12a crRNAs

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF706

Plasmid

#88037

Purpose

Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF706 (ZNF706 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF251

Plasmid

#101451

Purpose

Donor Vector containing ZNF251 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF251 (ZNF251 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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CSNK1G2 gRNA (BRDN0001162501)

Plasmid

#76988

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G2 (CSNK1G2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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