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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,692 results for: golden gate

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Showing: 2101 - 2120 of 5692 results

  1. pEPOR1CB0011

    Plasmid
    #117547
    Purpose
    Level 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117547-plasmid-map-sequence-id-228085

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  2. pEPOR1CB0009

    Plasmid
    #117545
    Purpose
    Level 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117545-plasmid-map-sequence-id-228083

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  3. pEPOR1CB0002

    Plasmid
    #117543
    Purpose
    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117543-plasmid-map-sequence-id-228068

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  15. AAV_Hs.TRAC_SEED_HDR_(TCRAFT2_makeTCR)

    Plasmid
    #256431
    Purpose
    CRISPR mediated HDR knock-in integration of TCRs into the Hs.TRAC locus using the SEED selection method, compatible with both makeTCR (BsmBI) and TCRAFT2 (SapI) high-throughput Golden Gate TCR cloning
    Depositor
    Michael Platten
    Publication
    Hamberger et al bioRxiv 2025.04.27.647198
    Type
    Empty backbone
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TRAC (knockin)
    Available since
    Oct. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    256431-plasmid-map-sequence-id-519033

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  18. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid
    #181790
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181790-plasmid-map-sequence-id-354811

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Showing: 2101 - 2120 of 5692 results