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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2101 - 2120 of 5523 results

  5. pEPQD0KN0751

    Plasmid
    #185628
    Purpose
    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI
    Depositor
    Nicola Patron
    Publication
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lacZ
    Use
    CRISPR and Synthetic Biology
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185628-plasmid-map-sequence-id-368556

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  9. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid
    #123182
    Purpose
    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Publication
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    Use
    Part for plant expression
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123182-plasmid-map-sequence-id-241413

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  13. pp-CBBC-Fluc

    Plasmid
    #232920
    Purpose
    Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.
    Depositor
    Philippe Mangeot
    Publication
    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232920-plasmid-map-sequence-id-464557

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  16. VN1897 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-Asf1B+rpoA-2GG

    Plasmid
    #235119
    Purpose
    Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion cloning to rpoA using BsaI based golden gate.
    Depositor
    Oscar Ramos
    Publication
    Guyot et al Comput Struct Biotechnol J. 2026 May 25;
    Type
    Empty backbone
    Use
    Synthetic Biology; Quantitative bacterial two-hyb…
    Tags
    cI_E34P
    Expression
    Bacterial
    Available since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    235119-plasmid-map-sequence-id-469387

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  18. SP_C3

    Plasmid
    #228159
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Publication
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q03178_PIR1_YEAST:0-18
    Use
    Synthetic Biology
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228159-plasmid-map-sequence-id-451652

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Showing: 2101 - 2120 of 5523 results