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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27,557 results for: nls

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Showing: 2101 - 2120 of 27557 results

  3. pEG302 22aa SunTag NtDRMcd nog

    Plasmid
    #115488
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115488-plasmid-map-sequence-id-237370

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  4. BAT-gal

    Plasmid
    #20889
    Depositor
    Stefano Piccolo
    Publication
    Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    7XTCF binding sites, Siamois minimal promoter, NLS-lacz
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    7 multimerized TCF-binding elements cloned upstre…
    Available since
    May 7, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. pMH186

    Plasmid
    #122837
    Purpose
    MS2-NLS (no stop codon) with GreenGate C-D flanking sequences
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-NLS, codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Tags
    Nuclear localisation signal
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122837-plasmid-map-sequence-id-239367

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  15. pSLQ10531

    Plasmid
    #239267
    Purpose
    Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines
    Depositor
    Stanley Qi
    Publication
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NLS-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239267-plasmid-map-sequence-id-475551

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  16. pGGF-NLS-NOS terminator-Hygromycin resistance

    Plasmid
    #246819
    Purpose
    A GreenGate entry vector containing SV40 NLS and Rex NLS followed by a Hygromycin resistance expression cassette
    Depositor
    Danhua Jiang
    Insert
    NLS-NOS terminator-Hygromycin resistance
    Use
    Greengate cloning entry vector
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    246819-plasmid-map-sequence-id-494251

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  20. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid
    #228557
    Purpose
    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus
    Depositor
    Neville Sanjana
    Publication
    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103
    Insert
    dRfxCas13d
    Use
    Lentiviral
    Tags
    NLS-HA
    Mutation
    R239A/H244A/R858A/H863A
    Available since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    228557-plasmid-map-sequence-id-456689

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Showing: 2101 - 2120 of 27557 results