We narrowed to 6,562 results for: nls

Showing: 2101 - 2120 of 6562 results

Results per page:

Sort results by:

C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Publication

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-QUASM1:GFPNLS-SV40pA

Plasmid

#155116

Purpose

Middle entry vector containing QUASM1 element upstream of GFP-NLS.

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Gateway middle entry vector

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC-Flag-CHES1L-dC-NLS

Plasmid

#51840

Purpose

Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

Depositor

Gerardo Ferbeyre

Publication

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-205

Available since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
T7-5'UTR-IS621-NLS-3'UTR-polyA (CJT503)

Plasmid

#248221

Purpose

IS621-bpNLS IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-IS621-NLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3omega2_[EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm]-[pNOS:KANAMYCIN:tNOS]

Plasmid

#254071

Purpose

3x YPet expression driven by EBSn

Depositor

Jose Alonso, Anna Stepanova

Publication

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm

Use

Synthetic Biology

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Plasmid

#217970

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

Depositor

Robert Piper

Publication

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Publication

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1205-Ai227 (TICF-nls-EGFP-ICF-tdTomato-WPRE)

Plasmid

#181761

Purpose

targeting vector

Depositor

Tanya Daigle

Insert

nls-tagged EGFP and tdTomato

Expression

Mammalian

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
G1333 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157838

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Publication

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
G1333 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157837

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Publication

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

Plasmid

#197031

Purpose

Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

Depositor

Xiangfeng Kong

Publication

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized denOsCas12f1-VPR

Expression

Mammalian

Mutation

D52R / T132R / D228A / D406A

Promoter

CBh, CMV, hU6

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

Plasmid

#108840

Purpose

Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA

Plasmid

#108842

Purpose

Insert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GAI,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA

Plasmid

#108836

Purpose

Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

Plasmid

#113437

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Publication

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

Plasmid

#107324

Purpose

Expresses SpCas9 fused to NmCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to NmCas9

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCambia2300-Aar1-BCPp-NLS-2xmCH_STR1p-cYFP-STR1g_STR2p-nYFP-STR2g

Plasmid

#254407

Purpose

BiFC plasmid with cYFP-STR and nYFP-STR2 expressed from their native promoters and MtBCP1-NLS- 2X mCherry.

Depositor

Maria Harrison

Publication

Ivanov et al New Phytol. 2026 Feb;249(4):1592-1604. d

Inserts

STR promoter-nYFP-STR_STR2 promoter-cYFP-STR2
M. truncatula BCP1 promoter - NLS-2X-mCherry

Expression

Plant

Promoter

MtBCP1 promoter and STR promoter

Available since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge