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-161+80pCalb2/mutE2F(-69)-like
Plasmid#66743Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationGCG (-69,-68,-67) to ATTPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
YC 3.6 Cameleon
Plasmid#58182PurposeLocalization: Cytosolic Ca Biosensor, Excitation: 433 / 515, Emission: 475 / 528DepositorTypeEmpty backboneUseTagsECFP and VenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
4MT-D3-CPV
Plasmid#58184PurposeLocalization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528DepositorTypeEmpty backboneUseTagsECFP and VenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
2MT-D2-CPV
Plasmid#58183PurposeLocalization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528DepositorTypeEmpty backboneUseTagsECFP and VenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.2 TDP-43 YFP
Plasmid#84911Purposeexpression of TDP-43 YFP in mammalian cellsDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-SV40
Plasmid#117811PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Mito-Car-GECO
Plasmid#100765PurposeLentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imagingDepositorInsertLenti-Mito-Car-GECO
UseLentiviralTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1: E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 5F-L YFP
Plasmid#84914PurposeMammalian expresion of TDP-43 5F-L YFPDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationF147L, F149L, F194L, F229L, F231LPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-CAAX-SV40
Plasmid#117809PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsCAAXExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-SV40
Plasmid#117805PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.BSD
Plasmid#57821PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.PAC
Plasmid#58329PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MEKDD
Plasmid#31202DepositorInsertMAPKK1 (MAP2K1 Human)
UseGateway donor vectorTagsExpressionMutationS218D S222DPromoterAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
cacnb4 (rat) in pMT2 vector
Plasmid#107426PurposeExpresses Calcium channel beta4 auxillary subunit in mammalian cellsDepositorInsertcacnb4 (Cacnb4 Rat)
UseTagsExpressionMammalianMutationPromoterAd MLPAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cherry-HA-KCC2
Plasmid#104077PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
UseTagsmCherry-HA tagExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD44-isoform1-CD4d3+4-bio
Plasmid#73098PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform1 with rat CD4d3+4-bioDepositorInsertCD44 antigen isoform 1 (CD44 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only
