We narrowed to 8,147 results for: Lif

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lentiGuide-SYNJ2BP-1

Plasmid

#92159

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-2

Plasmid

#92160

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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UAS-u(L)Cas9

Plasmid

#127385

Purpose

Expresses Cas9 at low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(L)-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-'S-mito

Plasmid

#158768

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-deltaTMD SYT7alpha

Plasmid

#179718

Purpose

Lentiviral expression of synaptic vesicle-targeted mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

Plasmid

#179719

Purpose

Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

Plasmid

#179722

Purpose

Lentiviral expression of lysosome-targeted mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

Plasmid

#179723

Purpose

Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) U6-SYT7 sgRNA 777 HaloTag

Plasmid

#179724

Purpose

U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

sgRNA and HaloTag

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) CamKII sf iGluSnFR S72A

Plasmid

#179726

Purpose

Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

iGluSnFR

Use

Lentiviral

Mutation

S72A

Availability

Academic Institutions and Nonprofits only

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pJR98

Plasmid

#187239

Purpose

CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

Expression

Bacterial

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROR2-V5/HIS

Plasmid

#205608

Purpose

Expression of human ROR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR2 receptor tyrosine kinase (ROR2 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGlucoSnFR-TS

Plasmid

#127952

Purpose

Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

Depositor

Gary Yellen

Article

Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

Insert

iGlucoSnFR-TS

Use

AAV

Tags

His6 and Xpress epitope

Expression

Mammalian

Promoter

CAG

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Alk3

Plasmid

#207614

Purpose

Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-mir-17-92

Plasmid

#64100

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN

Plasmid

#180647

Purpose

Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, has siRNA resistance to GAACAGUGUG…

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Sema4a-Fc-His

Plasmid

#72154

Purpose

Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4a (Sema4a Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-CAG-mNG2(1-10)

Plasmid

#206042

Purpose

Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette

Use

CRISPR and Synthetic Biology; Donor template

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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