We narrowed to 13,995 results for: Lif

1. CVS-N2c-nl.EGFP-SypGFP

   Plasmid

   #172380

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + SypEGFP
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFsynW SYB2 reporter

   Plasmid

   #197276

   Purpose

   Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYB2 and HaloTag (Vamp2 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   N/A

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ER-CaNAR2

   Plasmid

   #64732

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CYP450-CaNAR2
   

   Use

   Tags

   Cerulean3, N-terminal 27 amino acids from CYP450,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PresentER-MSIIFFLPL (GFP)

   Plasmid

   #102946

   Purpose

   PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Insert

   MSIIFFLPL (Serpinf1 Synthetic, Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF(UG) hSyn SYT7alpha-HaloTag

   Plasmid

   #179715

   Purpose

   Lentiviral expression of mouse Syt7 with C terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACS145

   Plasmid

   #204600

   Purpose

   Histone H3.3K36M-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.3 K36M (H3-3A Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.3 K36M - V5

   Expression

   Mammalian

   Mutation

   histone H3.3 K36M

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS-HsProm1s1-StayGold

   Plasmid

   #211333

   Purpose

   Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   AAV

   Tags

   StayGold

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-PM-CaNAR2

   Plasmid

   #64730

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   Lyn-CaNAR2
   

   Use

   Tags

   Cerulean3, N-terminal targeting sequence from Lyn…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABHD16A-mNG

   Plasmid

   #195157

   Purpose

   mammalian expression of ABHD16A tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid

    #202585

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFE-Reu-FI-prk

    Plasmid

    #162696

    Purpose

    Expresses R. eutropha Form IC rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    R. eutropha CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. σ1-mCherry

    Plasmid

    #186578

    Purpose

    Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP1S1 (AP1S1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

    Plasmid

    #166609

    Purpose

    Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-RT007-GCN4scFV-VB

    Plasmid

    #163387

    Purpose

    2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiGuide-RRBP1-1

    Plasmid

    #92156

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFE-Rs-FI-prk

    Plasmid

    #162697

    Purpose

    Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    R. spaeroides Form IC rubisco and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. VinTS-tCRMod-GGSGGS9

    Plasmid

    #111765

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-FLAG-APEX2-OPTN

    Plasmid

    #175760

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. UAS-u(M)Cas9

    Plasmid

    #127384

    Purpose

    Expresses Cas9 at medium level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(M)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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