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We narrowed to 5,185 results for: golden gate

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Showing: 2121 - 2140 of 5185 results

  6. pp-CBBC-Fluc

    Plasmid
    #232920
    Purpose
    Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.
    Depositor
    Philippe Mangeot
    Article
    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232920-plasmid-map-sequence-id-464557

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  7. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  8. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

    Plasmid
    #201537
    Purpose
    Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Insert
    Promoterless PuroR-P2A-miRFP720
    Expression
    Mammalian
    Promoter
    None
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201537-plasmid-map-sequence-id-401330

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  10. pJo55

    Plasmid
    #159623
    Purpose
    An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.
    Depositor
    Vasiliy Taranov
    Article
    Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    159623-plasmid-map-sequence-id-329054

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  14. pSB61 - pL0_V2 (CDS1)

    Plasmid
    #123183
    Purpose
    Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
    Use
    Part for plant expression
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Available Since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123183-plasmid-map-sequence-id-241416

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  19. pGA-red-maxi

    Plasmid
    #196337
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196337-plasmid-map-sequence-id-393160

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  20. pGA-blue

    Plasmid
    #196336
    Purpose
    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196336-plasmid-map-sequence-id-393157

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Showing: 2121 - 2140 of 5185 results