We narrowed to 12,861 results for: sequence

1. EF1a_LMX1A_P2A_Hygro_Barcode

   Plasmid

   #120457

   Purpose

   Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   LMX1A (LMX1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PECAM1-bio-His

   Plasmid

   #51624

   Purpose

   Expresses full-length Platelet endothelial cell adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PECAM1 (PECAM1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PPIB-bio-His

   Plasmid

   #53413

   Purpose

   Expresses full-length Peptidyl-prolyl cis-trans isomerase B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PPIB (PPIB Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_FOXA3_P2A_Hygro_Barcode

   Plasmid

   #120440

   Purpose

   Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   FOXA3 (FOXA3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_FOXA1_P2A_Hygro_Barcode

   Plasmid

   #120438

   Purpose

   Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   FOXA1 (FOXA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a_CDX2_P2A_Hygro_Barcode

   Plasmid

   #120432

   Purpose

   Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIREN-RetroQ-shN

   Plasmid

   #73665

   Purpose

   pSIREN-RetroQ vector containing control sequence N

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA Neg Control
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pB-Ric8b-Integration

   Plasmid

   #129457

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   Ric8b (Ric8b Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TrHKII-pGFPN3

    Plasmid

    #21921

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Truncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is the truncated human HKII cDNA sequence (l…

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

    Plasmid

    #197885

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD55-bio-His

    Plasmid

    #53152

    Purpose

    Expresses full-length CD55 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD55 (CD55 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. SNRPA_Full-length_pGEX

    Plasmid

    #135134

    Purpose

    Recombinant expression of SNRPA with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SNRPA (SNRPA Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. L309-myc-seipin-WT

    Plasmid

    #64061

    Purpose

    myc-tagged murine seipin (wild type); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. EF1a_KLF1_P2A_Hygro_Barcode

    Plasmid

    #120488

    Purpose

    Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF1 (KLF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. RBM4_2xRRM-ZNF_pGEX

    Plasmid

    #135154

    Purpose

    Recombinant expression of RBM4 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBM4 (RBM4 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sniper-Cas9

    Plasmid

    #138559

    Purpose

    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    Sniper-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

    Plasmid

    #197893

    Purpose

    Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

    Plasmid

    #184952

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Michael Bassik, Lacra Bintu, Patrick Hsu, Ami Bhatt

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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