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Rescue Septin 11 mRNA

Plasmid

#122025

Purpose

Rescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (CUG…

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-P2A-GFP

Plasmid

#247154

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β1β6-EGFP

Plasmid

#242826

Purpose

Encodes human integrin β1 chimera containing integrin β6 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 1, transcript variant 1A (ITGB1 Human)

Expression

Mammalian

Mutation

Integrin β1 chimera containing the cytoplasmic ta…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30-LaccID

Plasmid

#234652

Purpose

Expresses LaccID evolved variant in yeast

Depositor

Miguel Alcalde Galeote, Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID

Tags

HRV 3C protease site-GSG linker-8xHis tag

Expression

Yeast

Mutation

V11A, Q45R, N74S, R121S, I126V, D255G, L300V

Promoter

Gal1p

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-RNAgateDel-sgEIF3D

Plasmid

#222137

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

V291-F306 deletion

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521A-peGFP-N1

Plasmid

#228577

Purpose

Expresses mouse TMEM16F-I521A-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I521A plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521E-peGFP-N1

Plasmid

#228578

Purpose

Expresses mouse TMEM16F-I521E-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I521E plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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hA3A*-IscBn

Plasmid

#227146

Purpose

Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter

Depositor

Dali Li

Article

Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

Insert

bpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS

Mutation

D96R/E84R/V159R/H339A

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3331 MTK234-TetO-AtoBpegRNA

Plasmid

#226668

Purpose

MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

hU6-TetO-AtoBpegRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-TetO

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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