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pCAG-SRGAP2A-EGFP

Plasmid

#168501

Purpose

Expression of full length SRGAP2 with a C-termonal EGFP fusion

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attL1/L2 flanked MCS

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-cPHx3

Plasmid

#116855

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-YFP

Plasmid

#62630

Purpose

mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Long

Plasmid

#47395

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-sTagRFP-TUBA1B

Plasmid

#207765

Purpose

Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Str-Endo-KKXX_SBP-EGFP-LIMP2

Plasmid

#222323

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-mmCdh5pro (JDW 1239)

Plasmid

#224478

Purpose

Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Cdh5 promoter

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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0_T7-mScarlet3

Plasmid

#225927

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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