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pCFJ150-mex3

Plasmid

#80361

Purpose

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mbk2

Plasmid

#80360

Purpose

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1449_unc-73e_cDNA

Plasmid

#82363

Purpose

express unc-73e cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 e isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
shCDK4(1)

Plasmid

#73550

Purpose

shRNA(1) against CDK4

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
shCDK4(2)

Plasmid

#73551

Purpose

shRNA(2) against CDK4

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pET21a-Chaetomium thermophilum LIC

Plasmid

#74782

Purpose

encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Chaetomium thermophilum dynein light intermediate chain

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx4

Plasmid

#74343

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 4 (Dlx4 Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTD125 GFP:POP1

Plasmid

#71387

Purpose

localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

POP1 (POP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

ACT1

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
pTH1-SaSrtA-KQ

Plasmid

#64982

Purpose

Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108Q

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_wt-IRES-GFP

Plasmid

#26491

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-let-7 (~250nt flanking each side)

Use

Retroviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_2A-IRES-GFP

Plasmid

#26545

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-let-7 (~250nt flanking each side)

Use

Retroviral

Expression

Mammalian

Mutation

SD1 G->A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPA-IRES-GFP

Plasmid

#26547

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPA

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPB-IRES-GFP

Plasmid

#26548

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPB

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant B

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPC-IRES-GFP

Plasmid

#26549

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPC

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant C

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPD-IRES-GFP

Plasmid

#26551

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPD

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant D

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pSiP3

Plasmid

#21957

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pIS53 SERP1 3'UTR

Plasmid

#14499

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
3205 pCRII Mcl-1 fragment

Plasmid

#8757

Depositor

Stanley Korsmeyer

Article

Rinkenberger et al Genes Dev 2000 Jan 1;14(1):23-7.

Insert

Mcl-1 cDNA PCR fragment (Mcl1 Mouse)

Expression

Bacterial

Mutation

Base pairs 943-1223.

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pSB700 Cerulean-Weissman scaffold

Plasmid

#79377

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

scaffold

Use

CRISPR

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pSB700 Cerulean-Zhang2.0

Plasmid

#79378

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

zhang 2.0

Use

CRISPR

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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