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MSCV-KRAS-G12V-IRES-mCherry

Plasmid

#221025

Purpose

Fluorescent reporter for expressing the KRAS G12V mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12V (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Bsd-CMV>hCDK11A

Plasmid

#239212

Purpose

Expresses CDK11A in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin dependent kinase 11A (CDK11A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43 K181E GFP

Plasmid

#237549

Purpose

Expression of GFP-tag fused to mutant TDP-43 K181E

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

GFP-tagged TDP-43 K181E (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

TDP-43 K181E

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdICAM1-ST3-His

Plasmid

#210666

Purpose

Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD86-ST3-His

Plasmid

#210664

Purpose

Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD80-ST3-His

Plasmid

#210665

Purpose

Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-WT-IRES-mCherry

Plasmid

#221019

Purpose

Fluorescent reporter for expressing the KRAS wildtype CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS wildtype (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual wt p110α/p85α

Plasmid

#199302

Purpose

co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

Depositor

Peter Vogt

Article

Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

Insert

His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)

Tags

Avi and His

Expression

Insect

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

Plasmid

#85756

Purpose

All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Article

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Inserts

Csy4-T2A-SpRFN-P2A-GFP
gRNA

Use

CRISPR

Tags

Csy4, Csy4 recognition sequence, FokI fusion via …

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV and U6

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-MCL1

Plasmid

#202663

Purpose

Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43 ΔNTD GFP

Plasmid

#237546

Purpose

Expression of GFP-tag fused to mutant TDP-43 ΔNTD

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

GFP-tagged TDP-43 ΔNTD (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

TDP-43 1-90 a.a. deletion (TDP-43 35kDa)

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43 2M GFP

Plasmid

#237543

Purpose

Expression of GFP-tag fused to mutant TDP-43 2M

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

GFP-tagged TDP-43 2M (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

TDP-43 E14A and E17A

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43 3M GFP

Plasmid

#237544

Purpose

Expression of GFP-tag fused to mutant TDP-43 3M

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

GFP-tagged TDP-43 3M (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

TDP-43 E14A, E17A, E21A

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43 ΔCTD GFP

Plasmid

#237547

Purpose

Expression of GFP-tag fused to mutant TDP-43 ΔCTD

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

GFP-tagged TDP-43 ΔCTD (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

TDP-43 274-414 a.a. deletion

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mEos3.2GG

Plasmid

#203756

Purpose

Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

KRas c-term (KRAS Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD19-ST3-His_withSUMO

Plasmid

#223219

Purpose

Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

extracellular domain of B-lymphocyte antigen CD19 (CD19 Human)

Tags

Histag, SUMO, and Spytag

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-Neo-CMV-MCL1

Plasmid

#202668

Purpose

MCL1 overexpression vector with G418 resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-HTR6-RhoA sensor

Plasmid

#189614

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6 (HTR6 Human)

Tags

GFP and mScarlet

Expression

Mammalian

Promoter

Tet3G

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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