We narrowed to 23,097 results for: ache

1. pInducer20 CARD8-HA PAM L368G

   Plasmid

   #169985

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, L368G

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer20 CARD8-HA PAM R394E

   Plasmid

   #169987

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, R394E

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pInducer20 CARD8-HA PAM F405G

   Plasmid

   #169988

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, F405G

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pInducer20 CARD8-HA PAM F370G

   Plasmid

   #169986

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, F370G

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pInducer20 CARD8-HA PAM E274R

   Plasmid

   #169984

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, E274R

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221 CARD8 FIIND F405G + S297A

   Plasmid

   #169974

   Purpose

   pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   S297A, F405G

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 CARD8 FIIND E274R + S297A

   Plasmid

   #169968

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   S297A, E274R

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 CARD8 FIIND R394E + S297A

   Plasmid

   #169972

   Purpose

   pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   S297A, R394E

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221 CARD8 PAM E274R

   Plasmid

   #169977

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   PAM, E274R

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-HisA-HOXC6-2

    Plasmid

    #110365

    Purpose

    Mammalian expression of HisA-tagged HOXC6-2

    Depositor

    Carlos Moreno

    Article

    Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

    Insert

    HOXC6 (HOXC6 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    short isoform

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHTSHP-ShhN-Cys

    Plasmid

    #121136

    Purpose

    Expresses ShhN with a C-terminal cysteine residues in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, and MBP

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-191, with an addition…

    Promoter

    tac

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-PIAL2M-wt

    Plasmid

    #67909

    Purpose

    Inducible expression of PIAL2 in E.coli

    Depositor

    Andreas Bachmair

    Article

    Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

    Insert

    PIAL2 (AT5G41580 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    E281-A496

    Promoter

    P-lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid

    #58490

    Purpose

    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

    Insert

    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Glutamine

    Promoter

    Ubiquitin

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. SF-SON IRES Puro

    Plasmid

    #214402

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SF-SON IRES Tomato

    Plasmid

    #214403

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti CMV Puro DEST (w118-1) SDC3

    Plasmid

    #107501

    Purpose

    Expresses SDC3, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    SDC3 (codon optimized) (SDC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGFP-mHP1beta

    Plasmid

    #181901

    Purpose

    Fluorescently tagged HP1beta

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1beta (Cbx1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE2-EF1aL-Cre-IRES-NeoR-W

    Plasmid

    #126700

    Purpose

    lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    Cre-IRES
    

    Use

    Cre/Lox and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti CMV Puro DEST (w118-1) PHLDB1

    Plasmid

    #107503

    Purpose

    Expresses PHLDB1, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    PHLDB1 (PHLDB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nucleotide changes 1236 T>C, 1365 C>T, 1371…

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLgw ELK4-V5-EcoDam

    Plasmid

    #98607

    Purpose

    Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK4 (Elk4 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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