We narrowed to 10,582 results for: plasmids 101

1. Hy_pMT dati MCS circfirefly_900

   Plasmid

   #110116

   Purpose

   Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT dati MCS circfirefly_700

   Plasmid

   #110115

   Purpose

   Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT dati MCS circfirefly_500

   Plasmid

   #110114

   Purpose

   Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

   Plasmid

   #239293

   Purpose

   Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

   Depositor

   Anita Hopper

   Article

   Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

   Inserts

   T7 polymerase with NLS and P278L
   reverse tetracycline transactivator (rtTA)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains M2-SE-G72P mutations, described in Addge…

   Promoter

   TDH3 and tetracycline-responsive element (TRE) an…

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Pur-CAG-hM3Dq-mCherry

   Plasmid

   #80948

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM3Dq-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Pur-CAG-hM4Di-mCherry

   Plasmid

   #80947

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM4Di-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX nsp1 CoV2

   Plasmid

   #175512

   Purpose

   For protein expression of WT nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPseq-BFP-backbone

    Plasmid

    #85707

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid

    #169658

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127321

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

    Plasmid

    #219426

    Purpose

    Cre-dependent expression of luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

    Insert

    mNeonGreen, ISN-IE, ChRmine
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Expression

    Mutation

    Promoter

    EF-1alpha

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRISPseq-mCherry-backbone

    Plasmid

    #85708

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

    Plasmid

    #127864

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CoV-2-NSP3-c001

    Plasmid

    #173083

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CoV-2-NSP3-c002

    Plasmid

    #173084

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDK197

    Plasmid

    #134189

    Purpose

    Retroviral plasmid expressing LAP-Clasp C terminus

    Depositor

    Iain Cheeseman

    Article

    Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

    Insert

    LAP-Clasp C terminus (CLASP1 Human)
    

    Use

    Retroviral

    Tags

    LAP (GFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-rap1-K462-463-651R

    Plasmid

    #84564

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K462-463-651R

    Promoter

    none

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-rap1-K43-117-118R

    Plasmid

    #84565

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K43-117-118R

    Promoter

    none

    Available Since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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