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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 28,126 results for: STI;

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Showing: 10881 - 10890 of 28126 results

  4. SpCas9-HF1

    Plasmid
    #138556
    Purpose
    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138556-plasmid-map-sequence-id-274763

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  5. SpCas9-NRCH-ABE8e(V106W)

    Plasmid
    #198556
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198556-plasmid-map-sequence-id-388914

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  9. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  10. pCFJ1137

    Plasmid
    #84827
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84827-plasmid-map-sequence-id-163671

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Showing: 10881 - 10890 of 28126 results