We narrowed to 20,189 results for: ATO

1. pQCXIZ GFP PODXL 4K>R

   Plasmid

   #202420

   Purpose

   Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   K431/525/526/547R

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK297 (DHC1-mAID Hygro)

   Plasmid

   #140542

   Purpose

   DHC1 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK296 (DHC1-mAID Neo)

   Plasmid

   #140541

   Purpose

   DHC1 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1delWGR-L713F

   Plasmid

   #173941

   Purpose

   Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5 FLAG membrane-bound lipin-1 (TMD)

   Plasmid

   #120277

   Purpose

   Expression of ER/Golgi-tethered lipin-1 in mammalian cells

   Depositor

   Sirio Dupont

   Article

   Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

   Insert

   Lipin-1 (Lpin1 Mouse)
   

   Tags

   FLAG and in-frame fusion with the TMD Golgi-local…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-Enhancer-ZC3H12D_PromoterMUT

   Plasmid

   #153068

   Purpose

   ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutagenesis to disrupt 3 BHLHE40 binding sites. N…

   Promoter

   None

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-SAM-Puro

   Plasmid

   #198713

   Purpose

   For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   EF1a-MPH-Puro
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a and U6

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-SAM-Cre

   Plasmid

   #198714

   Purpose

   For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   Pgk-MPH-Cre
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   Pgk and U6

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ:K2P2.1(mTREK-1)Cryst S112C

   Plasmid

   #224780

   Purpose

   Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Expression

   Yeast

   Mutation

   K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …

   Promoter

   AOX1 promoter

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUb FLAG-Mts

    Plasmid

    #195065

    Purpose

    Expresses FLAG-tagged Drosophila mts protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Ubi-p63e

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUb FLAG-Pp2A-29B

    Plasmid

    #195066

    Purpose

    Expresses FLAG-tagged Drosophila Pp2A-29B protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Protein phosphatase 2A at 29B (Pp2A-29B Fly)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Ubi-p63e

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166133

    Purpose

    This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting cldn1 gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166134

    Purpose

    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_6xHis-PARP1delZn3-L713F

    Plasmid

    #173934

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. DHC1-mIAA7 Neo

    Plasmid

    #140543

    Purpose

    DHC1 tagging with mIAA7

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-Basic-ZC3H12D_PromoterMUT

    Plasmid

    #153067

    Purpose

    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutagenesis to disrupt 3 BHLHE4040 binding sites.…

    Promoter

    None

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5 FLAG membrane-bound lipin-1 (delta200)

    Plasmid

    #120278

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Tags

    FLAG and in-frame fusion with the delta200 Golgi-…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet-O-FUW-FOSL2-P2A-mCherry

    Plasmid

    #203860

    Purpose

    Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    FOSL2 (FOSL2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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