We narrowed to 4,240 results for: Alk

1. pLCKO_AAVS1_sgRNA_3

   Plasmid

   #155086

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LCV2_mCherry_AAVS1_sgRNA_4

   Plasmid

   #155106

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLCKO_AAVS1_sgRNA_4

   Plasmid

   #155087

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LCV2_AAVS1_sgRNA_4

   Plasmid

   #155090

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-2TKN-bp1

   Plasmid

   #133428

   Purpose

   Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   SREBF1 (SREBF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SREBP1a amino acids 1-50 fused to GST

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-Lrrc40-trunc-IRES-BFP

   Plasmid

   #117268

   Purpose

   Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Lrrc40 (Lrrc40 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The insert is truncated - deletion of bp 334-407 …

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA tagged SphI ELL2 M133, 138, 186ILeu

   Plasmid

   #127277

   Purpose

   Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminus

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met 133, 138, 186 to Ileu; HA inserted at SphI af…

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

   Plasmid

   #72609

   Purpose

   For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   changed threonine34 to alanine

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

   Plasmid

   #72610

   Purpose

   For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   changed serine33 to alanine

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

    Plasmid

    #72613

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Mutation

    Changed serine37 to alanine

    Promoter

    TRE tight promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid

    #72611

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33, threonine34 and serine37 to ala…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

    Plasmid

    #62576

    Purpose

    Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Luciferase

    Tags

    None

    Expression

    Mutation

    miR-206 binding site mutated (ACATTCCA --> AAC…

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223-PTK2

    Plasmid

    #23902

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-PIK3R1

    Plasmid

    #23696

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223-SYK

    Plasmid

    #23907

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SYK (SYK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBactin-AcGFP-128-425-Akap9

    Plasmid

    #196871

    Purpose

    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Akap9 (128-425) (Akap9 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-NTRK1

    Plasmid

    #23891

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NTRK1 (NTRK1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    V263L relative to NM_001012331

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223-ULK3

    Plasmid

    #23646

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ULK3 (ULK3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only