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R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

Plasmid

#170195

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Promoter

CMV51

Available Since

Nov. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-EWS-FLI1-KS

Plasmid

#237671

Purpose

For overexpression of mEGFP-EWS-FLI1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWS-FLI1-KS (EWSR1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ALCAM-p2A-E2Crimson

Plasmid

#83939

Purpose

Lentiviral vector expressing ALCAM-p2A-E2Crimson

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

ALCAM (ALCAM Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mScarlet-Giantin

Plasmid

#196872

Purpose

Expresion of Giantin-fused to mScarlet. Used as Golgi marker

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mScarlet-Giantin (GOLGB1 Synthetic, Human)

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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LCV2_LacZ_sgRNA_2

Plasmid

#155093

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-mCerulean

Plasmid

#154948

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

CaMKIIa(0.4)

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso A

Plasmid

#137720

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 long isoform (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-wtGATA6-3XAU1 neo

Plasmid

#72618

Purpose

Expresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Promoter

TRE Tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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LCV2_EGFP

Plasmid

#155098

Purpose

Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Bhlhe40-3xFLAG-IRES-BFP

Plasmid

#117263

Purpose

Retroviral overexpression of Bhlhe40 with a 3xFLAG

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Bhlhe40 (Bhlhe40 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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hSyn-GtACR2-ChRmine-tandem-eYFP

Plasmid

#192580

Purpose

AAV-vector for bidirectional optogenetic manipulation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

EYFP- soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SLEV_NS2B-GS-NS3

Plasmid

#203510

Purpose

Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

poly SLEV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso C

Plasmid

#137721

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoform

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 short isoform (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-EWSR1-repair-tempate

Plasmid

#237682

Purpose

For knock-in msfGFP to EWSR1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWSR1 (EWSR1 Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

Plasmid

#166010

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

Plasmid

#166011

Purpose

mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) (S )

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

D614G

Promoter

CMV51

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1

Plasmid

#72612

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight promoter

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLRC1-NEK10p:NEK10-3XFLAG

Plasmid

#137030

Purpose

Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

Depositor

David Sabatini

Article

Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

Insert

NIMA-like kinase 10 (NEK10 Human)

Use

Lentiviral

Tags

3XFLAG

Promoter

human NEK10 endogenous promoter

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNK115

Plasmid

#219745

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal luciferase

Use

Luciferase and Synthetic Biology

Mutation

I3S, N4T, F11L, I63T, T99P, T192S, A199P

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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