We narrowed to 2,612 results for: ubiquitin

1. pEF1a-Usp9x_CD-mCherry

   Plasmid

   #170380

   Purpose

   Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

   Depositor

   Miguel Ramalho-Santos

   Article

   Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

   Insert

   Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
   

   Use

   Tags

   mCherry-3xFlag

   Expression

   Mammalian

   Mutation

   Catalytic domain

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PHY23-phage-ubc-nls-ha-stdMCP-tdTomato

   Plasmid

   #164044

   Purpose

   Expression of stdMCP-tdTomato in mammalian cells

   Depositor

   Baohui Chen

   Article

   Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

   Insert

   stdMCP-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-StrepHA-SUMO2(Q87R)

   Plasmid

   #66868

   Purpose

   Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

   Depositor

   Ying Liu

   Article

   Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA and Strep

   Expression

   Mammalian

   Mutation

   Q87R

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3A-Gam

   Plasmid

   #119769

   Purpose

   Conversion of C to T monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
   

   Use

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUWR-Cad-Venus

   Plasmid

   #58328

   Purpose

   Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

   Depositor

   Denise Montell

   Article

   Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

   Insert

   Cad-Venus
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   poly-ubiquitin

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pYPQ146-ZmUbi-tRNA

   Plasmid

   #158404

   Purpose

   Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin 1

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ144-ZmUbi-tRNA

   Plasmid

   #158402

   Purpose

   Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin 1

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR RPS27A-3xFlag_IRES-GFP

   Plasmid

   #198390

   Purpose

   RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZmUBQ:AVRa1-V1

   Plasmid

   #132357

   Purpose

   Expression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotor

   Depositor

   Paul Schulze-Lefert

   Article

   Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

   Insert

   AVRa1-V1 ∆SP with stop codon
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pZmUBQ

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZmUBQ:AVRa1

    Plasmid

    #132356

    Purpose

    Expression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    AVRa1 ∆SP with stop codon
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLN599 (pFuGW-hUbCp-hSTE1)

    Plasmid

    #105206

    Purpose

    ubiquitin C promoter drives STE expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    hUbCp-hSTE1
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW-RapACR_T111C-EYFP

    Plasmid

    #119195

    Purpose

    Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR fast mutant T111C
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    changed threonine 111 to cysteine

    Promoter

    Ubiquitin

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET3a-hUb

    Plasmid

    #61937

    Purpose

    Expression of hUb in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    human ubiquitin (UBC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLenti-lxlplap

    Plasmid

    #45581

    Depositor

    Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Inserts

    Green fluorescent protein
    Placental alkaline phosphatase (ALPP Human)
    

    Use

    Lentiviral

    Tags

    Histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. FCIV1-GR

    Plasmid

    #72701

    Purpose

    Lentiviral expression of rat GR (2nd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

    Insert

    GR (Nr3c1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-Venus

    Expression

    Mammalian

    Mutation

    Promoter

    ubiquitin

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

16. PC7-709-28-1_CRBN-VPR

    Plasmid

    #200437

    Purpose

    Expresses the VPR transactivation domain linked to the CRBN E3 Ubiquitin Ligase

    Depositor

    Xue Gao

    Article

    Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

    Insert

    CRBN-VPR
    

    Use

    Tags

    CRBN, VPR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ141-ZmUbi-RZ-Aac.4

    Plasmid

    #158406

    Purpose

    Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-BD-cop1at(335-675)

    Plasmid

    #44973

    Depositor

    Thanos Halazonetis

    Article

    Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

    Insert

    BD-COP1 (COP1 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-334

    Promoter

    pCMV

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. UAS:bloSwitch

    Plasmid

    #108355

    Purpose

    UAS driven green to red switch reporter for B3 recombinase expression

    Depositor

    Harold Burgess

    Article

    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

    Insert

    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Zebrafish codon optimized

    Promoter

    lac

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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