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Plasmid#126576PurposeVector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBBR5pemIK
Plasmid#126554PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Green monkey (Chlorocebus sabaeus) sgRNA library
Pooled Library#178284PurposeGenome-wide CRISPR screening library targeting 20,991 genes in C. sabaeus, 84,963 total gRNAsDepositorExpressionMammalianSpeciesChlorocebus sabaeus (african green monkey)UseCRISPR and LentiviralAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromotersgkAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRT-KanR-FRT pA
Plasmid#82601Purpose3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selectionDepositorInsertFRT-Kan-FRT
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK-GW
Plasmid#126556PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
UseTagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
RT-5
Plasmid#220442PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)DepositorInsertPUF3 (puf-3 Nematode)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP553‐1
Plasmid#87240Purposegtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)DepositorInsertgtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R4 #BGV049
Plasmid#48943PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pREG(R1-R3) #BGV141
Plasmid#48958PurposeRetroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREG(R1-R4) #BGV059
Plasmid#48991PurposeRetroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R3 #BGV148
Plasmid#48942PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR3 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HMBP-3C-STAR.66-285
Plasmid#100094Purposeexpresses MBP-tagged STARD1 protein domain (residues 66-285)DepositorInsertSTAR protein (STAR Human)
UseTagsHis-tagged MBPExpressionBacterialMutationPromoterPtacAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168
Plasmid#133976Purposemammalian expression vector of Flag tagged wild type RNF168DepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
UseTagsExpressionBacterialMutationPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only