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Plasmid#190783PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, G154C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationG154CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(M165C)
Plasmid#190784PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationM165CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4B(S184C)
Plasmid#180026PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).DepositorInsertGB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS184CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C)
Plasmid#180027PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(363-393)
Plasmid#200314Purposebacterial expression of P53 CTDDepositorInsertp53(363-393) (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpGB1-CtNot2_X
Plasmid#147969PurposeBacterial Expression of CtNot2DepositorInsertCtNot2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2+pNOS_Red-F_NOSt
Plasmid#170887PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 LbCas12a (GB1439)
Plasmid#160573PurposeHuman codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).DepositorInsertLbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AsCas12a (GB1438)
Plasmid#160572PurposeHuman codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).DepositorInsertAsCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)
Plasmid#160617PurposeTU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD 5'UTR:YFP:Tnos (GB1483)
Plasmid#160557PurposePart 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)DepositorInsert5'UTR:YFP:Tnos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AlcA (GB1549)
Plasmid#160584PurposeEthanol inducible operatorDepositorInsertAlcA
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD DsRed-CT (GB1183)
Plasmid#160556PurposeDsRed for C-terminal fusionsDepositorInsertDsRed_CT
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)
Plasmid#75471PurposeTranscriptional unit for kanamycin resistance geneDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAgrobacterium tumefaciens Nopaline synthase promo…Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
Plasmid#78507PurposeConstruct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labelingDepositorInsertGads SH3 SH2 SH3 domain (GRAP2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
Plasmid#160611PurposeTranscriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:LbCas12a:T35s
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196)-Strep
Plasmid#196208PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetON-mEGFP-HMGB1-WT
Plasmid#194561PurposeDox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-WT
Plasmid#194543PurposeBacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)
Plasmid#160607PurposeTU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.DepositorInsertCUP2Gal4AD
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only