We narrowed to 1,142 results for: biotin

1. pEJS578_DD-dSpyCas9-mCherry-APEX2

   Plasmid

   #108570

   Purpose

   Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Insert

   dSpyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   APEX2, Destabilization domains, Flag, and mCherry…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MCS-TurboID MSCV-IRES-GFP

   Plasmid

   #207957

   Purpose

   Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

   Depositor

   Timothy Ley

   Article

   Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

   Insert

   TurboID
   

   Use

   Retroviral; Turboid biotin proximity ligase

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TurboID-MCS MSCV-IRES-GFP

   Plasmid

   #207958

   Purpose

   Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

   Depositor

   Timothy Ley

   Article

   Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

   Insert

   TurboID
   

   Use

   Retroviral; Turboid biotin proximity ligase

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Dead Streptavidin-SpyCatcher

   Plasmid

   #59547

   Purpose

   pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead-dloop-Streptavidin-SpyCatcher
   

   Use

   Tags

   N-terminally shortened SpyCatcher

   Expression

   Bacterial

   Mutation

   PolyAsp in L3/4 loop for ion-exchange

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLVX-UBE3B-BirA-Puro

   Plasmid

   #107352

   Purpose

   Expresses UBE3B fused to BirA in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B-BirA (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   Biotin ligase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. TIRAP-TAP_IRES_GFP

   Plasmid

   #52740

   Purpose

   Retroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFP

   Depositor

   Jonathan Kagan

   Article

   Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

   Insert

   Toll/IL-1 Receptor adaptor protein (TIRAP Human)
   

   Use

   Retroviral

   Tags

   3x HA, 3x TEV, and BirA target site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. arginine biosensor cpFLIPR

   Plasmid

   #205746

   Purpose

   Robust FRET biosensor enabling sensitive, accurate l-arginine measurement at physiological temp

   Depositor

   Colin Jackson

   Article

   Whitfield et al Protein Sci. 2015 Jun 9. doi: 10.1002/pr

   Insert

   cpFLIPR
   

   Use

   Unspecified

   Tags

   6 x Histidine purification tag (N-terminal), Biot…

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-BirA-UBE3B-Puro

   Plasmid

   #107351

   Purpose

   Expresses UBE3B fused to BirA in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   BirA-UBE3B (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   Biotin ligase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

   Plasmid

   #80325

   Purpose

   Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

   Depositor

   Daniel Schoenberg

   Article

   Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

   Insert

   PXDNL-003 (PXDNL Human)
   

   Use

   Tags

   Biotin

   Expression

   Mammalian

   Mutation

   H170A and H256A

   Promoter

   CMV

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid

    #167288

    Purpose

    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    APEX2 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pv9_TAF3_2xPHD-13XL-BASU

    Plasmid

    #179414

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3.2xPHD-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    TAF3.2xPHD-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pv9_CBX1_2xChromo-13XL-BASU

    Plasmid

    #179412

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1.2xChromo-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

    Plasmid

    #113898

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pet16b-Avi-ubiquitin_rbs_BirA

    Plasmid

    #134897

    Purpose

    Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Inserts

    Ubiquitin (UBB Human)
    BirA
    

    Use

    Tags

    10HIS-AviTag-PreScission(3C)protease

    Expression

    Bacterial

    Mutation

    Promoter

    RBS and T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pv9_CBX7_2xChromo-13XL-BASU

    Plasmid

    #179413

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7.2xChromo-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pv9_MBD1_MBD-WT-13XL-BASU

    Plasmid

    #179416

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1.MBD-WT-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5_FRB-pBirA-HA

    Plasmid

    #83415

    Purpose

    proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

    Insert

    FRB-pBirA-HA (MTOR Human, Synthetic)
    

    Use

    Tags

    HA and pBirA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge