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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,601 results for: sequence

Showing: 201 - 220 of 24601 results

  1. pDS24

    Plasmid
    #58952
    Purpose
    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93
    Insert
    Mitochondrially coded Arg8 protein
    Use
    Tags
    Expression
    Mutation
    Coding sequence synthetic in S. cerevisiae mitoch…
    Promoter
    n/a
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    Use
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available Since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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  6. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid
    #164266
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
    Depositor
    Nikolaus Rajewsky
    Article
    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    U6 promoter from W05B2.8
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164266-plasmid-map-sequence-id-325780

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  8. GB2890

    Plasmid
    #193133
    Purpose
    A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.
    Depositor
    Diego Orzaez
    Article
    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3
    Insert
    GB_SynP (A2) G1dG2b.1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    193133-plasmid-map-sequence-id-380393

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  12. mCherry_C1_miR21

    Plasmid
    #65970
    Purpose
    Expressed mCherry under the control of miR-21 (miR-21 complementary sequence in 3' UTR)
    Depositor
    Thomas Tuschl
    Insert
    miR-21 complementary sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    65970-plasmid-map-sequence-id-112135

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  13. pGL004

    Plasmid
    #215336
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hyper-pSpank
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215336-plasmid-map-sequence-id-426101

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  14. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

    Plasmid
    #102771
    Purpose
    "Activate neurons with 2p stimulation"
    Depositor
    Karel Svoboda
    Insert
    C1V1::FusionRed::Kv2.1
    Use
    AAV
    Tags
    FusionRed and Kv2.1 traficking domain
    Expression
    Mutation
    Promoter
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102771-plasmid-map-sequence-id-239474

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  15. pAG471 Lyn11-iFAST

    Plasmid
    #130817
    Purpose
    Expresses Lyn11-iFAST in mammalian cells (inner plasma membrane labeling)
    Depositor
    Arnaud Gautier
    Insert
    Lyn11-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130817-plasmid-map-sequence-id-254955

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  16. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  17. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid
    #171938
    Purpose
    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins
    Depositor
    David Berson
    Insert
    Apex2, mRuby2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171938-plasmid-map-sequence-id-338493

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  18. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid
    #171937
    Purpose
    Expresses APEX2 linked to tau for labeling microtubules
    Depositor
    David Berson
    Insert
    tau-APEX2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171937-plasmid-map-sequence-id-338490

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  19. pCCG_nit9_without_linker

    Plasmid
    #228411
    Purpose
    Expression of NIT-9 without linkage region in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    deletion of the linkage region
    Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228411-plasmid-map-sequence-id-459451

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  20. pCCG_nit9fl_reverse

    Plasmid
    #228410
    Purpose
    Expression of NIT-9 with reversed domains in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9 domains reversed
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    domains reoriented
    Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228410-plasmid-map-sequence-id-459450

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Showing: 201 - 220 of 24601 results