Addgene

We narrowed to 4,414 results for: ARA-2

Showing: 2181 - 2200 of 4414 results

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-15nmEML-mRFP

Plasmid

#231177

Purpose

Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

15nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-INTS3-C

Plasmid

#128642

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 C-termini (INTS3 Human)

Use

Tags

His tag on N and C-terminal

Expression

Bacterial

Mutation

INTS3 C-termini (514–1042 aa)

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

Plasmid

#197961

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-vCasphi-2

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

Plasmid

#197962

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-nCasphi-2

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_MCS_version2

Plasmid

#197950

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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PX458-sgTSC2

Plasmid

#128098

Purpose

CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

sgRNA against human TSC2 (TSC2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDMS151A_Tet-On-3G

Plasmid

#191577

Purpose

ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

Tet-On(R) 3G

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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AMPK-SPARK

Plasmid

#226255

Purpose

phases-of-separation based activity reporter of AMPK

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

AMPK-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD/HisB-Medium-FT

Plasmid

#31917

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Use

Tags

His

Expression

Bacterial

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

araB

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_MCS_version1

Plasmid

#197937

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMRBad-Z-C-wtBlc

Plasmid

#168256

Purpose

C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly

Depositor

Jens Meiler

Article

Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

Insert

Leucine Zipper + C-fragment of wtBlc

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP-VPR

Plasmid

#181907

Purpose

Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9-VPR

Use

CRISPR

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx26-IRES-GCaMP6s

Plasmid

#188236

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJB2 (GJB2 Human)

Use

Lentiviral

Tags

GCaMP6s

Expression

Mammalian

Mutation

Promoter

Tight TRE promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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A1-LCD+7R+12D

Plasmid

#178678

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

Depositor

Tanja Mittag

Article

Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

Insert

A1-LCD+7R+12D (HNRNPA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

seven Arg residues added, twelve Glu residues add…

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL2

Plasmid

#163611

Purpose

Overexpression of human BCL2

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

BCL2 (BCL2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1alpha

Plasmid

#181900

Purpose

Fluorescently tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-GFP-mHP1alpha

Plasmid

#181912

Purpose

Bacterial protein expression vector for GFP-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMECP2-GFP

Plasmid

#181904

Purpose

Fluorescently tagged MECP2, GFP

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

MECP2 (Mecp2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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