We narrowed to 11,490 results for: ENA

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pJJB1340

Plasmid

#218593

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Mutation

Pex22(1-36)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSKT001

Plasmid

#218594

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1357

Plasmid

#218595

Purpose

Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex11

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1461

Plasmid

#218587

Purpose

Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Erg12

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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MDH1_KW

Plasmid

#224145

Purpose

IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Thomas et al Biochim Biophys Acta Proteins Proteom. 2

Insert

MDH-1 (F46E10.10 Nematode)

Tags

Ala-intein-CBD

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ248

Plasmid

#217494

Purpose

Mammlian expression of DpFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc guide RNA scaffold

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Cterm_His

Plasmid

#220485

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Nterm_His

Plasmid

#220478

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Nterm_His

Plasmid

#220479

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Nterm_His

Plasmid

#220480

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1377_Nterm_His

Plasmid

#220481

Purpose

heterologous expression of PA1377 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAN052

Plasmid

#220046

Purpose

Mammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tags

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

FLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ234

Plasmid

#217492

Purpose

Mammlian expression of DpFnuc for genome editing

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_A

Plasmid

#185803

Purpose

A synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the ABI1 promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(ABI1)+-86MP+erGFP

Expression

Plant

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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6xABRE_R

Plasmid

#185804

Purpose

a synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoter

Depositor

Jose Dinneny

Article

Wu et al Plant Physiol. 2018 Aug;177(4):1650-1665

Insert

6xABRE(RD29A)+-86MP+erGFP

Expression

Plant

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3-NLS

Plasmid

#203489

Purpose

Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Gateway entry vector

Tags

Nuclear localization signal and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-MTS-jAspSnFR3-mRuby3

Plasmid

#203490

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Gateway entry vector

Tags

His-tag, Mitochondrial localization signal, and m…

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9

Plasmid

#193862

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Type

Empty backbone

Tags

GFP11, Hisx6, and R9

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-T2A-EGFP

Plasmid

#188271

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-PlmCasX-T2A-EGFP

Plasmid

#188273

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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