We narrowed to 9,602 results for: FIE
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Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV human IRS-1 CAAX
Plasmid#11238DepositorInsertIRS-1 (IRS1 Human)
UseTagsCAAXExpressionMammalianMutationC-terminal prenylation motif makes IRS-1 localize…PromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AK1-His
Plasmid#124135PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAdenylate kinase (AK1 Chicken)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-SIINFEKL
Plasmid#174601Purposeexpresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitopeDepositorInsertmembrane bound CD19, SIINFEKL (ovalbumin epitope)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-Rac1-DN
Plasmid#109582PurposeMultisite gateway vector for 3' tagging with dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F263-OKT3
Plasmid#190680PurposeEncodes an EGFRvIII-CD3 BITE protein (F263 clone)DepositorInsertAnti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLC-217
Plasmid#73163PurposeTemplate plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.DepositorInsertPrhaB-relE (relE E. coli (bacteria))
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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actc1b-mKate2-Rac1
Plasmid#109529PurposeMuscle specific zebrafish expression of mKate2 fused to Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1-DN
Plasmid#109530PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTO149_RFP_CauNEO
Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
UseTagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP1 (catalytic domain)
Plasmid#16356DepositorInserthuman SENP1 catalytic domain (SENP1 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 419 to 644 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUASp-GBP-Tbro
Plasmid#179995PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
G10 Cas9 MDC123
Plasmid#59187PurposeG10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoterG10Available sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70
Plasmid#62897PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_SLK172
Plasmid#224395PurposeFor expression of the type I CBASS system encoded by Escherichia coli SLK172DepositorInsertEcCdnD_4TM
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F265-OKT3
Plasmid#190679PurposeEncodes an EGFRvIII-CD3 BITE protein (F265 clone)DepositorInsertAnti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
7Gli:GFP
Plasmid#110494Purposereporter of Hedgehog signaling activityDepositorInsert7 repeats of the Gli binding site GAACACCCA
UseLentiviralTagsExpressionMammalianMutationPromoter7 repeats of Gli biding siteAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only