We narrowed to 10,872 results for: Kars;

1. pcDNA3.1 phiC31

   Plasmid

   #68310

   Purpose

   In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

   Depositor

   Konrad Basler

   Article

   Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

   Insert

   phiC31
   

   Use

   Rna transcription

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPomyces-AsCas12j-2

   Plasmid

   #191655

   Purpose

   All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

   Depositor

   Fong Tian Wong

   Article

   Tan et al bioRxiv 2021.10.28.465406

   Insert

   AsCas12j-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEvoltac EcW-TGA-h9

   Plasmid

   #218765

   Purpose

   encodes EcWRS-h9 and EcW-tRNA-TGA

   Depositor

   Abhishek Chatterjee

   Article

   Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

   Insert

   EcWRS-h9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S8A, V144S, V146A

   Promoter

   tacI

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRE recombinase

   Plasmid

   #62730

   Purpose

   Expresses Cre recombinase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-8xHis-Ub

   Plasmid

   #107392

   Purpose

   Mammalian expression 8xHis-Ub

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   8 tandem repeats of 6xHis-Ub (See comments below)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTPGI_dCas9_VP64

   Plasmid

   #49013

   Purpose

   encodes yeast-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   pTPGI (galactose+aTc inducible)

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC-LYCipi

   Plasmid

   #53279

   Purpose

   Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSKI015

   Plasmid

   #11570

   Depositor

   Detlef Weigel

   Article

   Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

   Insert

   CaMV 35S enhancers
   

   Use

   Activation tagging

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDIRE

    Plasmid

    #26745

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    iCre expression cassette
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HPV16 E6 TCR

    Plasmid

    #122727

    Purpose

    Express HPV16 E6 TCR

    Depositor

    Christian Hinrichs

    Article

    Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

    Insert

    HPV16 E6 TCR
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSKI074

    Plasmid

    #11571

    Depositor

    Detlef Weigel

    Article

    Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

    Insert

    CaMV 35S enhancers
    

    Use

    Activation tagging

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDsRed1-N1 RanGAP

    Plasmid

    #13386

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBMN HA

    Plasmid

    #188645

    Purpose

    This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-Rap1b

    Plasmid

    #118325

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

    Insert

    Rap1b (RAP1B Bovine)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-TetO-Sox9

    Plasmid

    #41080

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO + minimal CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCZGY2727

    Plasmid

    #193330

    Purpose

    Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

    Depositor

    Yishi Jin

    Article

    Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

    Insert

    Hygromycin resistance
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Prps-0

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHSV-TK

    Plasmid

    #165801

    Purpose

    Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-2xFLAG-SREBP-2

    Plasmid

    #26807

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    L7V, G76S, K395R and I482L

    Promoter

    CMV

    Available since

    July 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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